Auditory and vestibular research : methods and protocols /: methods and protocols. (2016)

• Record Type: Book
• Further Information: Note: Edited by Bernd Sokolowski.
• Editors: Sokolowski, Bernd
• Contents: Helios® Gene Gun-Mediated Transfection of the Inner Ear Sensory Epithelium: Recent Updates -- Tol2-Mediated Delivery of miRNAs to the Chicken Otocyst using Plasmid Electroporation -- A Rapid, Cost-Effective Method of Recombinant Adeno-Associated Virus Preparation Enables Efficient Gene Transfer to the Developing Mouse Inner Ear -- Generation of Noninvasive, Quantifiable, Orthotopic Animal Models for NF2-Associated Schwannoma and Meningioma -- RNA Extraction from Xenopus Auditory and Vestibular Organs for Molecular Cloning and Expression Profiling with RNA-Seq and Microarrays -- Yeast Two-Hybrid Screening to Test for Protein-Protein Interactions in the Auditory System -- Multiplexed Isobaric Tagging Protocols for Quantitative Mass Spectrometry Approaches to Auditory Research -- Protein Quantification of the Developing Cochlea by Mass Spectrometry -- Ultrastructural Identification and Colocalization of Interacting Proteins in the Murine Cochlea by Post-Embedding Immunogold Transmission Electron Microscopy -- Surface Plasmon Resonance (SPR) Analysis of Binding Interactions of Inner-Ear Proteins -- The Single-Molecule Approach to Membrane Protein Stoichiometry -- Visualization of Live Cochlear Stereocilia at a Nanoscale Resolution Using Hopping Probe Ion Conductance Microscopy -- Design and Construction of a Cost-Effective Spinning Disk System for Live Imaging of Inner Ear Tissue -- Neuroanatomical Tracing Techniques: History, State of the Art, and Future Developments -- Detection of Excitation and Inhibition in the Auditory System Using Fluorescence Immunohistochemistry and High Resolution Fluorescence Microscopy -- Rapid and Semi-Automated Extraction of Neuronal Cell Bodies and Nuclei from Electron Microscopy Image Stacks.-Organotypic Culture of the Mouse Cochlea from Embryonic Day 12 to the Neonate -- Quantifying Spiral Ganglion Neurite and Schwann Behavior on Micropatterned Polymer Substrates -- The Use of Human Wharton's Jelly Cells for Cochlear Tissue Engineering -- Nanotechnology in Auditory Research: Membrane Electromechanics in Hearing -- Overview of Nanoparticle Based Delivery for Treatment of Inner Ear Disorders -- Development of Cell-based High-Throughput Chemical Screens for Protection Against Cisplatin-induced Ototoxicity.-Profiling Specific Inner Ear Cell Types Using Cell Sorting Techniques -- Optical Coherence Tomography to Measure Sound-Induced Motions Within the Mouse Organ of Corti In Vivo -- Method for Dissecting the Auditory Epithelium (Basilar Papilla) in Developing Chick Embryos -- Whole Cell Patch Clamp Recording of Mouse and Rat Inner Hair Cells in the Intact Organ of Corti -- Glass Probe Stimulation of Hair Cell Stereocilia -- A Walkthrough of Nonlinear Capacitance Measurement of Outer Hair Cells -- In Vitro Functional Assessment of Adult Spiral Ganglion Neurons (SGNs) -- Mapping Auditory Circuits with Photostimulation of Caged Glutamate. .
• Edition: [Second edition]
• Publisher Details: New York, NY : Humana Press
• Publication Date: 2016
• Extent: 1 online resource (xvi, 547 pages), illustrations (some color)
• Subjects: 612.8/58; Medicine; Vestibular apparatus; Hearing disorders; Equilibrium (Physiology); Equilibrium (Physiology); Hearing disorders; Vestibular apparatus; Hearing Disorders; Medical -- Genetics; Medical genetics; Human genetics; Science -- Life Sciences -- Biology -- Molecular Biology; Medical research; Electronic books; Electronic books
• Languages: English
• ISBNs: 9781493936151; 1493936158
• Related ISBNs: 9781493936137
• Notes: Note: Online resource; title from PDF title page (SpringerLink, viewed June 10, 2016).
• Access Rights: Legal Deposit; Only available on premises controlled by the deposit library and to one user at any one time; The Legal Deposit Libraries (Non-Print Works) Regulations (UK).
• Access Usage: Restricted: Printing from this resource is governed by The Legal Deposit Libraries (Non-Print Works) Regulations (UK) and UK copyright law currently in force.