Lentiviral vectors and exosomes as gene and protein delivery tools. (2016)

• Record Type: Book
• Further Information: Note: Edited by Maurizio Federico.
• Editors: Federico, Maurizio
• Contents: Construction of Modular Lentiviral Vectors for Effective Gene Expression and Knockdown -- Development of Inducible Molecular Switches Based on All-In-One Lentiviral Vectors Equipped With Drug Controlled FLP Recombinase -- Production Of Retrovirus-Based Vectors In Mildly Acidic Ph Conditions -- Optimized Lentiviral Transduction Protocols by use of a Poloxamer Enhancer, Spinoculation and Scfv-Antibody Fusions To VSV-G -- Transduction of Murine Hematopoietic Stem Cells with Tetracycline-Regulated Lentiviral Vectors -- Introduction of Shrnas, Mirnas or Antagomirs into Primary Human Liver Cells Through Lentiviral Vectors -- Production and Concentration of Lentivirus for Transduction of Primary Human T Cells -- Generating Transgenic Mice by Lentiviral Transduction of Spermatozoa Followed by In Vitro Fertilization and Embryo Transfer -- The LAM-PCR Method to Sequence LV Integration Sites -- Conditional Rnai using the Lentiviral GLTR System -- Lentiviral Vectors for the Engineering of Implantable Cells Secreting Recombinant Antibodies -- Transient Expression of Green Fluorescent Protein in Integrase-Defective Lentiviral Vector Transduced 293T Cell Line -- Intrastriatal Delivery of Integration-Deficient Lentiviral Vectors in a Rat Model of Parkinson's Disease -- Development of Lentiviral Vectors for Targeted Integration and Protein Delivery -- Biogenesis and Functions of Exosomes and Extracellular Vesicles -- Generation, Quantification, and Tracing of Metabolically Labeled Fluorescent Exosomes -- Cardiac Myocyte Exosome Isolation -- Incorporation of Heterologous Proteins in Engineered Exosomes -- Exosome-Mediated Targeted Delivery of Mirnas.
• Publisher Details: New York, NY : Humana Press
• Publication Date: 2016
• Extent: 1 online resource (xi, 276 pages), illustrations (some color)
• Subjects: 579.2/569; Medicine; Lentiviruses -- Laboratory manuals; Lentiviruses; Lentivirus; Human genetics; Medical -- Genetics; Medical genetics; Electronic books; Laboratory manuals; Laboratory manuals; Laboratory Manuals
• Languages: English
• ISBNs: 9781493937530; 1493937537; 1493937510; 9781493937516
• Related ISBNs: 9781493937516; 1493937510
• Notes: Note: Includes bibliographical references and index.; Note: Online resource; title from PDF title page (SpringerLink, viewed June 23, 2016).
• Access Rights: Legal Deposit; Only available on premises controlled by the deposit library and to one user at any one time; The Legal Deposit Libraries (Non-Print Works) Regulations (UK).
• Access Usage: Restricted: Printing from this resource is governed by The Legal Deposit Libraries (Non-Print Works) Regulations (UK) and UK copyright law currently in force.