Synthetic mRNA : production, introduction into cells and physiological consequences /: production, introduction into cells and physiological consequences. ([2016])

• Record Type: Book
• Further Information: Note: Edited by Robert E. Rhoads.
• Other Names: Rhoads, Robert E, 1944-
• Contents: Synthetic mRNA: Production, Introduction into Cells, and Physiological Consequences -- Synthetic Capped mRNAs for Cap-Specific Photo-Crosslinking Experiments -- Enzymatic Modification of 5'-Capped RNA and Subsequent Labeling by Click Chemistry -- Preparation of Functional, Fluorescently Labeled mRNA Capped with Anthraniloyl-m7GpppG -- Intronless b-Globin Reporter: A Tool for Studying Nuclear RNA Stability Elements -- Synthetic mRNA with Superior Properties that Mimics the Intracellular Fates of Natural Histone mRNA -- Engineering WT1-Encoding mRNA to Increase Translational Efficiency in Dendritic Cells -- Electroporation of Alphavirus RNA Translational Reporters into Fibroblastic and Myeloid Cells as a Tool to Study the Innate Immune System -- GMP-Grade mRNA Electroporation of Dendritic Cells for Clinical Use -- Large Scale mRNA Transfection of Dendritic Cells by Electroporation in Continuous Flow Systems -- FLT3 Ligand as a Molecular Adjuvant for Naked RNA Vaccines -- Transfecting Human Monocytes with RNA -- In Vitro Synthesis, Delivery and Bioavailability of Exogenous mRNA in Gene Transfer Mediated byPiggyBac Transposition -- Transfection of Human Keratinocytes with Nucleoside-Modified mRNA Encoding CPD-Photolyase to Repair DNA Damage -- Delivery of Synthetic mRNA Encoding FOXP3 Antigen into Dendritic Cells for Inflammatory Breast Cancer Immunotherapy -- Immune Monitoring using mRNA-Transfected Dendritic Cells -- Transfection of Tumor-Infiltrating T Cells with mRNA Encoding CXCR2 -- mRNA Electroporation of Dendritic Cells with WT1, Survivin and TriMix (A Mixture of caTLR4, CD40L, and CD70) -- Redirecting T Cell Specificity Using T Cell Receptor Messenger RNA Electroporation -- Measuring Hematocrit in Mice Injected with In Vitro-Transcribed Erythropoietin mRNA -- Genetic Modification of Human Pancreatic Progenitor Cells through Modified mRNA.
• Publisher Details: New York : Humana Press Springer
• Publication Date: 2016
• Extent: 1 online resource (xii, 320 pages), illustrations (some color)
• Subjects: 572.8/8; Medicine; Messenger RNA -- Laboratory manuals; Messenger RNA; RNA, Messenger -- chemical synthesis; Cell Engineering -- methods; Cell Differentiation -- genetics; Human genetics; Medical -- Genetics; Medical genetics; Electronic books; Laboratory manuals; Laboratory manuals; Laboratory Manuals
• Languages: English
• ISBNs: 9781493936250; 1493936255; 1493936239; 9781493936236
• Related ISBNs: 9781493936236
• Notes: Note: Includes bibliographical references and index.; Note: Online resource; title from PDF of title page (Springer Protocols, viewed May 31, 2016).
• Access Rights: Legal Deposit; Only available on premises controlled by the deposit library and to one user at any one time; The Legal Deposit Libraries (Non-Print Works) Regulations (UK).
• Access Usage: Restricted: Printing from this resource is governed by The Legal Deposit Libraries (Non-Print Works) Regulations (UK) and UK copyright law currently in force.