Baculovirus and insect cell expression protocols. (2016)

• Record Type: Book
• Further Information: Note: Edited by David W. Murhammer.
• Editors: Murhammer, David W
• Contents: Useful Tips, Widely Used Techniques And Quantifying Cell Metabolic Behavior -- Introduction to Baculovirus Molecular Biology -- Baculovirus Transfer Vectors -- Recombinant Baculovirus Isolation -- Gene Expression In Mammalian Cells Using Bacmam, A Modified Baculovirus System -- Available Lepidopteran Insect Cell Lines -- Lepidopteran Insect Cell Line Isolation from Insect Tissue -- Development Of Serum-Free Media For Lepidopteran Insect Cell Lines -- Routine Maintenance And Storage Of Lepidopteran Insect Cell -- Small-Scale Production Of Recombinant Proteins Using The Baculovirus Expression Vector System -- Recombinant Protein Production In Large Scale Agitated Bioreactors Using The Baculovirus Expression Vector System -- Protein Expression in Insect and Mammalian Cells Using Baculoviruses in Wave Bioreactors -- Protein Production With Recombinant Baculoviruses in Lepidopteran Larvae -- Production Of Virus-Like Particles For Vaccination -- Alternative Strategies for Expressing Multi-Component Protein Complexes in Insect Cells -- Transforming Lepidopteran Insect Cells for Continuous Recombinant Protein Expression -- Stable Drosophila Cell Lines: An Alternative Approach to Exogenous Protein Expression -- Transforming Lepidopteran Insect Cells for Improved Protein Processing and Expression -- Introduction To The Use Of Baculoviruses As Biological Insecticides -- Baculovirus Insecticide Production In Insect Larvae -- Evaluation Of The Insecticidal Efficacy Of Wild Type And Recombinant Baculoviruses -- Evaluating Baculovirus Infection Using Green Fluorescent Protein and Variants -- Tubular Bioreactor for Probing Baculovirus Infection and Protein Production -- Gene Silencing In Insect Cells Using Rnai -- Using the Baculovirus/Insect Cell System to Study Apoptosis -- Generation of Envelope-Modified Baculoviruses for Gene Delivery into Mammalian Cells.
• Publisher Details: New York, NY : Humana Press
• Publication Date: 2016
• Extent: 1 online resource (xii, 508 pages), illustrations (some color)
• Subjects: 579.2/436; Life sciences; Baculoviruses -- Genetics -- Laboratory manuals; Genetic engineering -- Laboratory manuals; Virus-vector relationships -- Laboratory manuals; Insect cell biotechnology -- Laboratory manuals; Baculoviruses -- Genetics; Genetic engineering; Insect cell biotechnology; Virus-vector relationships; Baculoviridae -- genetics; Gene Expression; Genetic Vectors; Genetic Techniques; Medical -- Microbiology; Medical microbiology & virology; Microbiology; Medical virology; Science -- Life Sciences -- Biology -- Microbiology; Microbiology (non-medical); Electronic books; Laboratory manuals; Electronic books
• Languages: English
• ISBNs: 9781493930432; 1493930435
• Related ISBNs: 9781493930425; 1493930427
• Notes: Note: Includes bibliographical references and index.; Note: Online resource; title from PDF title page (SpringerLink, viewed February 4, 2016).
• Access Rights: Legal Deposit; Only available on premises controlled by the deposit library and to one user at any one time; The Legal Deposit Libraries (Non-Print Works) Regulations (UK).
• Access Usage: Restricted: Printing from this resource is governed by The Legal Deposit Libraries (Non-Print Works) Regulations (UK) and UK copyright law currently in force.