Kinase screening and profiling : methods and protocols /: methods and protocols. ([2016])

• Record Type: Book
• Further Information: Note: Edited by Hicham Zegzouti and Said A. Goueli.
• Editors: Zegzouti, Hicham; Goueli, Said A
• Contents: 1. HTRF Kinase Assay Development and Methods in Inhibitor Characterization Yong Jia, Mari Manuia, and Jose Juarez2. Application of Eukaryotic Elongation Factor-2 Kinase (eEF-2K) for Cancer Therapy: Expression, Purification, and High-Throughput Inhibitor ScreeningClint D. J. Tavares, Ashwini K. Devkota, Kevin N. Dalby, and Eun Jeong Cho3. Recombinant Kinase Production and Fragment Screening by NMR SpectroscopyByeonggu Han and Hee-Chul Ahn4. Bioluminescence Methods for Assaying Kinases in Quantitative High-Throughput Screening (qHTS) Format Applied to Yes1 Tyrosine Kinase, Glucokinase and PI5P4Kα Lipid KinaseMindy I. Davis, Douglas S. Auld, and James Inglese5. Using Bioluminescent Kinase Profiling Strips to Identify Kinase Inhibitor Selectivity and PromiscuityHicham Zegzouti, Jacquelyn Hennek, and Said A. Goueli6. Measuring Activity of Phosphoinositide Lipid Kinases Using a Bioluminescent ADP-Detecting AssayAndrew W. Tai and Jolanta Vidugiriene7. A High-Throughput Radiometric Kinase AssayKrisna C. Duong-Ly and Jeffrey R. Peterson8. A High Content Assay to Screen for Modulators of EGFR Function Christophe Antczak and Hakim Djaballah9. Monitoring Protein Kinase Expression and Phosphorylation in Cell Lysates with Antibody MicroarraysHong Zhang, Xiaoqing Shi, and Steven Pelech10. From Enzyme to Whole Blood: Sequential Screening Procedure for Identification and Evaluation of p38 MAPK InhibitorsSilke M. Bauer, Jakub M. Kubiak, Ulrich Rothbauer, and Stefan Laufer11. Genetically Encoded Fluorescent Indicators to Visualize Protein Phosphorylation in Living CellsMoritoshi Sato and Yoshio Umezawa12. Characterization of an Engineered Src Kinase to Study Src Signaling and BiologyLeanna R. Gentry, Andrei V. Karginov, Klaus M. Hahn, and Channing J. Der13. Screening One-Bead-One-Compound Peptide Libraries for Optimal Kinase SubstratesThi B. Trinh and Dehua Pei14. Determination of the Substrate Specificity of Protein Kinases with Peptide Micro- and MacroarraysShenshen Lai, Dirk F.H. Winkler, Hong Zhang, and Steven Pelech15. Rapid Identification of Protein Kinase Phosphorylation Site Motifs Using Combinatorial Peptide LibrariesChad J. Miller and Benjamin E. Turk.
• Publisher Details: New York, NY : Humana Press Springer
• Publication Date: 2016
• Copyright Date: 2016
• Extent: 1 online resource (x, 223 pages), illustrations (some color)
• Subjects: 572/.792; Life sciences; Phosphotransferases; Phosphotransferases; Phosphotransferases; Biochemistry; Science -- Life Sciences -- Biochemistry; Proteins; Electronic books; Electronic books
• Languages: English
• ISBNs: 9781493930739; 1493930737; 1493930729; 9781493930722
• Related ISBNs: 9781493930722
• Notes: Note: Includes bibliographical references and index.
• Access Rights: Legal Deposit; Only available on premises controlled by the deposit library and to one user at any one time; The Legal Deposit Libraries (Non-Print Works) Regulations (UK).
• Access Usage: Restricted: Printing from this resource is governed by The Legal Deposit Libraries (Non-Print Works) Regulations (UK) and UK copyright law currently in force.