Mitochondrial DNA : methods and protocols /: methods and protocols. (©2016)

• Record Type: Book
• Further Information: Note: Edited by Matthew McKenzie.
• Other Names: McKenzie, Matthew
• Contents: Comprehensive Mitochondrial Genome Analysis By Massively Parallel Sequencing -- Analysis Of Translesion DNA Synthesis By The Mitochondrial DNA Polymerase g -- Quantification Of DNA Damage By Real-Time Qpcr -- Quantitation Of Mitochondrial DNA Deletions Via Restriction Digestion/Long-Range Single Molecule PCR -- A Single-Cell Resolution Imaging Protocol Of Mitochondrial DNA Dynamics In Physiopathology, Mtrip, Which Also Evaluates Sublethal Cytotoxicity -- Scalable Isolation Of Mammalian Mitochondria For Nucleic Acid And Nucleoid Analysis -- Tracking Mitochondrial DNA In Situ -- Analysis Of Replicating Mitochondrial DNA By In Organello Labeling And Two-Dimensional Agarose Gel Electrophoresis -- Translation And Assembly Of Radiolabelled Mitochondrial DNA-Encoded Protein Subunits From Cultured Cells And Isolated Mitochondria -- Analysis Of Mitochondrial DNA Copy Number And Its Regulation Through DNA Methylation Of POLGA -- Engineered Mtzfns For Manipulation Of Human Mitochondrial DNA Heteroplasm -- Generation Of Xenomitochondrial Embryonic Stem Cells For The Production Of Live Xenomitochondrial Mice -- Import Of Fluorescent RNA Into Mitochondria Of Living Cells -- Purification And Comparative Assay Of The Human Mitochondrial Replicative DNA Helicase -- Expression And Purification Of Mitochondrial RNA Polymerase And Transcription Factor A From Drosophila Melanogaster -- Purification And Comparative Assay Of Human Mitochondrial Single-Stranded DNA-Binding Protein -- Biolayer Interferometry: A Novel Method To Elucidate Protein- Protein And Protein- DNA Interactions In The Mitochondrial DNA Replisome.
• Edition: 3rd ed
• Publisher Details: New York : Humana Press Springer
• Publication Date: 2016
• Copyright Date: 2016
• Extent: 1 online resource (xi, 234 pages), illustrations
• Subjects: 572.8/69; Medicine; Mitochondrial DNA -- Laboratory manuals; Mitochondrial DNA; DNA, Mitochondrial; Genetic Techniques; Human genetics; Medical -- Genetics; Medical genetics; Electronic books; Laboratory manuals; Electronic books
• Languages: English
• ISBNs: 9781493930401; 1493930400
• Related ISBNs: 9781493930395; 1493930397
• Notes: Note: Includes bibliographical references and index.
• Access Rights: Legal Deposit; Only available on premises controlled by the deposit library and to one user at any one time; The Legal Deposit Libraries (Non-Print Works) Regulations (UK).
• Access Usage: Restricted: Printing from this resource is governed by The Legal Deposit Libraries (Non-Print Works) Regulations (UK) and UK copyright law currently in force.