Cytoskeleton : methods and protocols /: methods and protocols. (2016)

• Record Type: Book
• Further Information: Note: Edited by Ray H. Gavin.
• Other Names: Gavin, Ray H
• Contents: Imaging the cytoskeleton in live and fixed cells. Long-term live cell imaging of cell migration : effects of pathogenic fungi on human epithelial cell migration / Torsten Wöllert and George M. Langford -- Live-cell imaging of mitochondria and the actin cytoskeleton in budding yeast / Ryo Higuchi-Sanabria, Theresa C. Swayne, Istvan R. Boldogh, and Liza A. Pon -- Imaging of the actin cytoskeleton and mitochondria in fixed budding yeast cells / Ryo Higuchi-Sanabria, Theresa C. Swayne, Istvan R. Boldogh, and Liza A. Pon -- Imaging of the cytoskeleton using live and fixed Drosophila tissue culture cells / Derek A. Applewhite, Christine A. Davis, Eric R. Griffis, and Omar A. Quintero -- Imaging cytoskeleton components by electron microscopy / Tatyana Svitkina -- Purification and localization of intraflagellar transport particles and polypeptides / Roger D. Sloboda -- Fluorescence imaging of the cytoskeleton in plant roots / Julia Dyachok, Ana Paez-Garcia, Cheol-Min Yoo, Karuppaiah Palanichelvam, and Elison B. Blancaflor -- Microtubules in plant cells : strategies and methods for immunofluorescence, transmission electron microscopy, and live cell imaging / Katherine Celler, Miki Fujita, Eiko Kawammura, Chris Ambrose, Klaus Herburger, Andreas Holzinger, and Geoffrey O. Wasteneys. Cytoskeleton dynamics. Basic methods to visualize actin filaments in vitro using fluorescence microscopy for observation of filament severing and bundling / Shoichiro Ono -- An in vitro model system to test mechano-microbiological interactions between bacteria and host cells / Luís Carlos Santos, Emilia Laura Munteanu, and Nicolas Biais -- Reconstitution of a minimal actin cortex by coupling actin filaments to reconstituted membranes / Sven K. Vogel -- Use of nanobodies to localize endogenous cytoskeletal proteins and to determine their contribution to cancer cell invasion by using an ECM degradation assay / Isabel Van Audenhove and Jan Gettemans -- Actin-dynamics in plant cells : the function of actin-perturbing substances : Jasplakinolide, Chondramides, Phalloidin, Cytochalasins and Latrunculins / Andreas Holzinger and Kathrin Blaas. Cell and organelle motility. Quantitative motion analysis in two and three dimensions / Deborah J. Wessels, Daniel F. Lusche, Spencer Kuhl, Amanda Scherer, Edward Voss, and David R. Soll -- Measurement of cell motility using microgrooved substrates / James H.-C. Wang, Guangyi Zhao, and Bin Li -- The study of cell motility by cell traction force microscopy (CTFM) / James H.-C. Wang, Guangyi Zhao, and Bin Li -- Melanosome motility in fish retinal pigment epithelial (RPE) cells / Christina King-Smith -- Analysis of stem cell motility in vivo based on ommunodetection of planarian neoblasts and tracing of BrdU-labeled cells after partial irradiation / Junichi Tasaki, Chichiro Uchiyama-Tasaki, and Labib Rouhana -- Chemotaxis : under agarose assay / Derrick Brazill. Genetic and proteomic protocols. Functional analysis of actin-binding proteins in the central nervous system of Drosophila / Qi He and Christopher Roblodowski -- Proteomic analysis of cytoskeleton proteins in fish / Michael Gotesman, Simon Menanteau-Ledouble, and Mansour El-Mabouli -- Using a handheld gene gun for genetic transformation of Tetrahymena thermophila / Michael Gotesman and Selwyn A. Williams -- Proteomic tools for the analysis of cytoskeleton proteins / Michael Scarpati, Mary Ellen Heavner, Eliza Wiech, and Shaneen Singh -- Homology modeling procedures for cytoskeletal proteins of Tetrahymena and other ciliated protists / Giovanni J. Pagano, Linda A. Hufnagel, and Roberta S. King.
• Edition: 3rd ed
• Publisher Details: New York, NY : Humana Press Springer
• Publication Date: 2016
• Extent: 1 online resource (xiii, 431 pages), illustrations
• Subjects: 571.6/54; Life sciences; Cytoskeleton -- Laboratory manuals; Cytoskeletal proteins -- Laboratory manuals; Cytoskeletal proteins; Cytoskeleton; Cytoskeleton; Cytoskeletal Proteins; Cytology; Science -- Life Sciences -- Biology -- Molecular Biology; Cellular biology (cytology); Laboratory manuals; Laboratory manuals; Electronic books; Laboratory Manuals
• Languages: English
• ISBNs: 9781493931248; 1493931245
• Related ISBNs: 9781493931231; 1493931237
• Notes: Note: Includes bibliographical references and index.
• Access Rights: Legal Deposit; Only available on premises controlled by the deposit library and to one user at any one time; The Legal Deposit Libraries (Non-Print Works) Regulations (UK).
• Access Usage: Restricted: Printing from this resource is governed by The Legal Deposit Libraries (Non-Print Works) Regulations (UK) and UK copyright law currently in force.