Hedgehog signaling protocols. (2015)

• Record Type: Book
• Further Information: Note: Edited by Natalia A. Riobo.
• Other Names: Riobo, Natalia A
• Contents: Controlled Delivery of Sonic Hedgehog with a Heparin-Based Coacervate -- In Vivo Imaging of Hedgehog Transport in Drosophila Epithelia -- Modeling Hedgehog Signaling Through Flux-Saturated Mechanism -- Evaluating the Activity of Smoothened Toward G Proteins Using [35S] Guanosine 5{u2019}-(3-O-thio)triphosphate ([35S]GTPÞS) -- Analysis of Smoothened Phosphorylation and Activation in Cultured Cells and Wing Discs of Drosophila -- Investigation of Protein-Protein Interactions and Conformational Changes in Hedgehog Signaling Pathway by FRET -- Luciferase Reporter Assays to Study Transcriptional Activity of HH Signaling in Normal and Cancer Cells -- Measuring Expression Levels of Endogenous Gli Genes by Immunoblotting and Real-Time PCR -- Quantitative Immunoblotting of Endogenous Hedgehog Pathway Components -- Measuring Gli2 Phosphorylation by Selected Reaction Monitoring Mass Spectrometry -- Rapid Screening of Gli2/3 Mutants Using the Flp-In System -- Insights into Gli Factors Ubiquitylation Methods -- Determination of Acetylation of the Gli Transcription Factors -- Efficient Detection of Indian Hedgehog During Endochondral Ossification by Whole Mount Immunofluorescence -- Methods for Detection of Ptc1-Driven LacZ Expression in Adult Mouse Skin -- Determination and Analysis of Cellular Metabolic Changes by Non-Canonical Hedgehog Signaling.
• Edition: 2nd ed
• Publisher Details: New York : Humana Press Springer
• Publication Date: 2015
• Extent: 1 online resource (xvi, 200 pages), illustrations (some color)
• Subjects: 571.7/4; Life sciences; Cellular signal transduction -- Research -- Methodology; Transcription factors -- Research -- Methodology; Cellular signal transduction -- Research -- Methodology; Hedgehog Proteins; Microbiological Techniques; Signal Transduction; Gene Expression Regulation; Biochemistry; Science -- Life Sciences -- Biochemistry; Proteins; Electronic books; Laboratory Manuals
• Languages: English
• ISBNs: 9781493927722; 1493927728
• Related ISBNs: 9781493927715; 149392771X; 9781493927715
• Notes: Note: Includes bibliographical references and index.; Note: Online resource; title from PDF title page (SpringerProtocols, viewed July 20, 2015).
• Access Rights: Legal Deposit; Only available on premises controlled by the deposit library and to one user at any one time; The Legal Deposit Libraries (Non-Print Works) Regulations (UK).
• Access Usage: Restricted: Printing from this resource is governed by The Legal Deposit Libraries (Non-Print Works) Regulations (UK) and UK copyright law currently in force.