CRISPR : methods and protocols /: methods and protocols. (2015)

• Record Type: Book
• Further Information: Note: Edited by Magnus Lundgren, Emmanuelle Charpentier, Peter C. Fineran.
• Editors: Lundgren, Magnus; Charpentier, Emmanuelle; Fineran, Peter C
• Contents: Investigating CRISPR RNA Biogenesis and Function using RNA-seq -- In vitro Co-reconstitution of Cas Protein Complexes -- Analysis of CRISPR pre-crRNA Cleavage -- Annotation and Classification of CRISPR-Cas Systems -- Computational Detection of CRISPR/crRNA Targets -- High-throughput CRISPR typing of Mycobacterium tuberculosis Complex and Salmonella enterica Serotype Typhimurium -- Spacer-based Macroarrays for CRISPR Genotyping -- Analysis of crRNA using Liquid Chromatography Electrospray Ionisation Mass Spectrometry (LC ESI MS) -- Rapid Multiplex Creation of Escherichia coli Strains Capable of Interfering with Phage Infection through CRISPR -- Exploring CRISPR Interference by Transformation with Plasmid Mixtures: Identification Of Target Interference Motifs in Escherichia coli -- Electrophoretic Mobility Shift Assay of DNA and CRISPR-Cas Ribonucleoprotein Complexes -- Expression and Purification of the CMR (Type III-B) Complex in Sulfolobus solfataricus -- Procedures for Generating CRISPR Mutants With Novel Spacers Acquired from Viruses Or Plasmids -- Archaeal Viruses of the Sulfolobales: Isolation, Infection, and CRISPR Spacer Acquisition -- Using the CRISPR-Cas system to Positively Select Mutants In Genes Essential for its Functionl -- Analysis of Nuclease Activity of Cas1 Proteins Against Complex DNA Substrates -- Characterizing Metal-Dependent Nucleases of CRISPR-Cas prokaryotic Adaptive Immunity Systems -- Cas3 Nuclease-Helicase Activity Assays -- Chemical and Enzymatic Footprint Analyses of R-loop Formation by Cascade-crRNA Complex -- Creation and Analysis of a Virome: Using CRISPR Spacers -- Targeted Mutagenesis in Zebrafish using CRISPR RNA-guided Nucleases -- Precise Genome Editing of Drosophila with CRISPR RNA-guided Cas9 -- Targeted Transcriptional Repression in Bacteria using CRISPR Interference (CRISPRi).
• Publisher Details: New York, NY : Humana Press
• Publication Date: 2015
• Extent: 1 online resource (xiv, 366 pages), illustrations (some color)
• Subjects: 572.8; Medicine; CRISPR (Genetics); Genetic regulation; Bacterial genetics; CRISPR (Genetics); Bacterial genetics; Genetic regulation; CRISPR-Associated Proteins -- analysis; Gene expression; Medical -- Genetics; Medical genetics; Laboratory manuals; Electronic books; Laboratory manuals; Laboratory Manuals
• Languages: English
• ISBNs: 9781493926879; 1493926861; 9781493926862
• Related ISBNs: 149392687X; 9781493926862
• Notes: Note: Includes bibliographical references and index.; Note: Includes bibliographical references and index (pages 362-366).; Note: Online resource; title from PDF title page (SpringerLink, viewed May 22, 2015).
• Access Rights: Legal Deposit; Only available on premises controlled by the deposit library and to one user at any one time; The Legal Deposit Libraries (Non-Print Works) Regulations (UK).
• Access Usage: Restricted: Printing from this resource is governed by The Legal Deposit Libraries (Non-Print Works) Regulations (UK) and UK copyright law currently in force.