ELISA : methods and protocols /: methods and protocols. (2015)

• Record Type: Book
• Further Information: Note: Edited by Robert Hnasko.
• Editors: Hnasko, Robert
• Contents: The Biochemical Properties of Antibodies and their Fragments -- Hybridoma Technology -- Affinity Purification of Antibodies -- Bio conjugation of Antibodies to Horseradish Peroxidase (HRP). -- Indirect ELISA -- Direct ELISA -- A Double-Sandwich ELISA for Identification of Monoclonal Antibodies Suitable for Sandwich Immunoassays -- ELISpot and DC-ELISspot Assay to Measure Frequency of Antigen-Specific IFNg Secreting Cells -- The Western Blot -- Development of Flow Through Assay for Detection of Antibodies to Taenia solium Using Protein-A Colloidal Gold Conjugate as a Probe.-Multiplexed Microsphere Suspension Array-Based Immunoassays -- Multiplex Immunoassay: A Planar Array on a Chip using the MagArray{u2122} Technology -- Lateral Flow Immunoassay -- Immuno-PCR Assay for Sensitive Detection of Proteins in Real Time -- In-situ Proximity Ligation Assay (PLA) -- Tyramide Signal Amplification for Immunofluorescent Enhancement -- Correlative microscopy for Localization of Proteins In Situ: Pre-Embedding Immuno-Electron Microscopy using FluoroNanogold, Gold Enhancement and Low-Temperature Resin -- tiplex ELISA using Oligonucleotide Tethered Antibodies -- Gas Plasma Surface Chemistry for Biological Assays.
• Publisher Details: New York : Humana Press
• Publication Date: 2015
• Extent: 1 online resource (x, 216 pages), illustrations (some color)
• Subjects: 616.07/56; Medicine; Enzyme-linked immunosorbent assay -- Laboratory manuals; Enzyme-linked immunosorbent assay; Enzyme-Linked Immunosorbent Assay -- methods; Immunoassay -- methods; Immunoglobulins -- biosynthesis; Biomedicine; Immunology; Immunology; Medical -- Immunology; Immunology; Electronic books; Laboratory manuals; Electronic books
• Languages: English
• ISBNs: 9781493927425; 1493927426; 1493927418; 9781493927418
• Related ISBNs: 9781493927418
• Notes: Note: Includes bibliographical references and index.; Note: Print version record.
• Access Rights: Legal Deposit; Only available on premises controlled by the deposit library and to one user at any one time; The Legal Deposit Libraries (Non-Print Works) Regulations (UK).
• Access Usage: Restricted: Printing from this resource is governed by The Legal Deposit Libraries (Non-Print Works) Regulations (UK) and UK copyright law currently in force.