RNA remodeling proteins : methods and protocols /: methods and protocols. (©2015)

• Record Type: Book
• Further Information: Note: Edited by Marc Boudvillain.
• Editors: Boudvillain, Marc
• Contents: Key Points to Consider When Studying RNA Remodeling by Proteins -- Happy Birthday: 25 years of DEAD-box Proteins -- In vivo Cross-linking Followed by polyA Enrichment to Identify Yeast mRNA Binding Proteins -- Dynamics of the Spb4 Interactome Monitored by Affinity Purification -- Using EMOTE to Map the Exact 5'-ends of Processed RNA on a Transcriptome-wide Scale -- Cellular Localization of RNA Degradation and Processing Components in Escherichia coli -- Recombineering Applications for the Mutational Analysis of Bacterial RNA-binding Proteins and Their Sites of Action -- Determination of RNA Chaperone Activity Using an Escherichia coli Mutant -- Biochemical Characterization of G4 Quadruplex Telomerase RNA Unwinding by the RNA Helicase RHAU -- ATPase Site Configuration of the RNA Helicase DbpA Probed by ENDOR Spectroscopy -- Bioinformatics and Biochemical Methods to Study the Structural and Functional Elements of DEAD-box RNA Helicases -- Measuring Helicase Inhibition of the DEAD-box Protein Dbp2 by Yra1 -- A FRET-based, Continuous Assay for the Helicase Activity of DEAD-box Proteins -- A Fluorescence-Based Screening Assay for Identification of Hepatitis C Virus NS3 Helicase Inhibitors and Characterization of Their Inhibitory Mechanism -- Mechanisms of HCV NS3 Helicase Monitored by Optical Tweezers -- Constructing a Magnetic Tweezers to Monitor RNA Translocation at the Single-molecule Level -- Probing RNA Translocases with DNA -- Monitoring RNA Unwinding by the Transcription Termination Factor Rho From Mycobacterium Tuberculosis -- Characterization of the Mechanisms of Transcription Termination by the Helicase Sen1 -- Characterization of TRAP-mediated Regulation of the B. subtilis trp Operon Using in vitro Transcription and Transcriptional Reporter Fusions in vivo -- Single-molecule FRET Characterization of RNA Remodeling Induced by an Antitermination Protein -- Fluorescence Reporters for Hfq Oligomerization and RNA Annealing -- Fluorescence Anisotropy-based Salt-titration Approach to Characterize Protein-Nucleic Acid Interactions -- Probing Hfq:RNA Interactions with Hydroxyl Radical and RNase Footprinting -- Purification of Eukaryotic Exoribonucleases Following Heterologous Expression in Bacteria and Analysis of Their Biochemical Properties by in vitro Enzymatic Assays -- Tips and Tricks to Probe the RNA-degrading Activities of Hyperthermophilic Archaeal {OCLCbr#DD}-CASP Ribonucleases.
• Publisher Details: New York, NY : Humana Press Springer
• Publication Date: 2015
• Copyright Date: 2015
• Extent: 1 online resource (xiv, 470 pages), illustrations (some color)
• Subjects: 572.8/8; Life sciences; RNA -- Laboratory manuals; Proteins -- Laboratory manuals; Proteins; RNA; Nucleic Acids; Amino Acids, Peptides, and Proteins; RNA Nucleotidyltransferases; Nucleic Acids, Nucleotides, and Nucleosides; Nucleotidyltransferases; Chemicals and Drugs; Phosphotransferases; Transferases; Enzymes; Enzymes and Coenzymes; RNA; RNA Helicases; Proteins; Science -- Life Sciences -- Biochemistry; Proteins; Biochemistry; Electronic books; Laboratory manuals; Electronic books
• Languages: English
• ISBNs: 9781493922147; 1493922149; 1493922130; 9781493922130
• Related ISBNs: 9781493922130
• Notes: Note: Includes bibliographical references and index.; Note: Online resource; title from PDF title page (SpringerLink, viewed January 22, 2015).
• Access Rights: Legal Deposit; Only available on premises controlled by the deposit library and to one user at any one time; The Legal Deposit Libraries (Non-Print Works) Regulations (UK).
• Access Usage: Restricted: Printing from this resource is governed by The Legal Deposit Libraries (Non-Print Works) Regulations (UK) and UK copyright law currently in force.