Plant functional genomics : methods and protocols /: methods and protocols. (2015)

• Record Type: Book
• Further Information: Note: Edited by Jose M. Alonso, Anna N. Stepanova.
• Editors: Alonso, Jose M; Stepanova, Anna N
• Contents: Epigenome Profiling of Specific Plant Cell Types Using a Streamlined INTACT Protocol and ChIP-seq -- Whole-Genome DNA Methylation Profiling with Nucleotide Resolution -- High-Throughput Nuclease-Mediated Probing of RNA Secondary Structure in Plant Transcriptomes -- Genome-Wide Mapping of DNase I Hypersensitive Sites in Plants -- Characterization of in-vivo DNA-Binding Events of Plant Transcription Factors by ChIP-seq: Experimental Protocol and Computational Analysis -- Identification of Direct Targets of Plant Transcription Factors Using the GR Fusion Technique -- Ribosome Profiling: A Tool for Quantitative Evaluation of Dynamics in mRNA Translation -- Tissue-Specific Gene Expression Profiling by Cell Sorting -- Translating Ribosome Affinity Purification (TRAP) Followed by RNA Sequencing Technology (TRAP-SEQ) for Quantitative Assessment of Plant Translatomes -- Rapid Immunopurification of Ribonucleoprotein Complexes of Plants -- Metabolomic Profiling of Plant Tissues -- Targeted Plant Genome Editing via the CRISPR/Cas9 Technology -- QTL Mapping Using High-Throughput Sequencing -- Quantitating Plant MicroRNA-Mediated Target Repression Using a Dual-Luciferase Transient Expression System -- Persistent Virus-Induced Gene Silencing in Asymptomatic Accessions of Arabidopsis -- A User's Guide to the Arabidopsis T-DNA Insertion Mutant Collections -- Genome-Wide Association Mapping in Plants Exemplified for Root Growth in Arabidopsis thaliana -- Tilling By Sequencing -- SHOREmap v3.0: Fast and Accurate Identification of Causal Mutations from Forward Genetic Screens -- Software-Assisted Stacking of Gene Modules Using GoldenBraid 2.0 DNA-Assembly Framework -- Ligation Independent Cloning for Plant Research -- Gene Functional Analysis Using Protoplast Transient Assays -- Descriptive vs. Mechanistic Network Models in Plant Development in the Post-Genomic Era -- Analysis and Visualization of RNA-Seq Expression Data Using RStudio, Bioconductor, and Integrated Genome Browser -- Constructing Simple Biological Networks for Understanding Complex High-Throughput Data in Plants.
• Publisher Details: New York, NY : Humana Press
• Publication Date: 2015
• Extent: 1 online resource (xv, 532 pages), illustrations (some color)
• Subjects: 581.3/5; Life sciences; Plant genetics -- Laboratory manuals; Functional genomics -- Laboratory manuals; Botany; Functional genomics; Plant genetics; Science -- Life Sciences -- Botany; Botany & plant sciences; Electronic books; Laboratory manuals; Electronic books
• Languages: English
• ISBNs: 9781493924448; 1493924443; 1493924435; 9781493924431
• Related ISBNs: 9781493924431
• Notes: Note: Includes bibliographical references and index.; Note: Online resource; title from PDF title page (SpringerLink, viewed March 20, 2015).
• Access Rights: Legal Deposit; Only available on premises controlled by the deposit library and to one user at any one time; The Legal Deposit Libraries (Non-Print Works) Regulations (UK).
• Access Usage: Restricted: Printing from this resource is governed by The Legal Deposit Libraries (Non-Print Works) Regulations (UK) and UK copyright law currently in force.