Salamanders in regeneration research : methods and protocols /: methods and protocols. (2015)

• Record Type: Book
• Further Information: Note: Edited by Anoop Kumar, András Simon.
• Editors: Kumar, Anoop; Simon, András, 1969-
• Contents: Variation in salamanders : an essay on genomes, development, and evolution / Jeremy P. Brockes -- Maintaining eastern newts (Notophthalmus viridescens) for regeneration research / Hans-Georg Simon and Shannon Odelberg -- Housing and maintenance of Ambystoma mexicanum, the Mexican axolotl / Johanna E. Farkas and James R. Monaghan -- Husbandry of Spanish ribbed newts (Pleurodeles waltl) / Alberto Joven, Matthew Kirkham, and András Simon -- Maintaining plethodontid salamanders in the laboratory for regeneration studies / Claudia Marcela Arenas, Andrea Gómez-Molina, and Jean Paul Delgado -- Newt lens transdifferentiation : from lentectomy to immuno-FISH / Nobuyasu Maki -- Studying newt brain regeneration following subtype specific neuronal ablation / Matthew Kirkham and Alberto Joven -- The accessory limb model : an alternative experimental system of limb regeneration / Tetsuya Endo, David M. Gardiner, Aki Makanae, and Akira Satoh -- High-efficiency electroporation of the spinal cord in larval axolotl / Aida Rodrigo Albors and Elly M. Tanaka -- Pseudotyped retroviruses for infecting axolotl / Tzu-Hsing Kuo and Jessica L. Whited -- Thyroxine-induced metamorphosis in the axolotllotl (Ambystoma mexicanum) / Peggy S. Coots and Ashley W. Seifert -- Generation of aneurogenic larvae by parabiosis of salamander embryos / Anoop Kumar and Jean Paul Delgado -- In vivo modulation and quantification of microRNAs during axolotl tail regeneration / Jami R. Erickson and Karen Echeverri -- Derivation and long-term culture of cells from newt adult limbs and limb blastemas / Patrizia Ferretti and Anoop Kumar -- Culture and transfection of axolotl cells / Jean-François Denis, Fadi Sader, Patrizia Ferretti, and Stéphane Roy -- Isolation and culture of neurospheres from the adult newt brain / Liyakath Ali Shahul Hameed and András Simon -- Methods for axolotl blood collection, intravenous injection, and efficient leukocyte isolation from peripheral blood and the regenerating limb / Ryan J. Debuque and James W. Godwin -- Assessing cardiomyocyte proliferative capacity in the newt heart and primary culture / Hans-Georg Simon and Shannon Odelberg -- Long-term organ cultures of newt hearts / Tanja Piatkowski and Thomas Braun -- In vitro preparation of newt inner ear sensory epithelia as a model for repair and regeneration / Ruth R. Taylor -- Transgenesis in axolotl (Ambystoma mexicanum) / Shahryar Khattak and Elly M. Tanaka -- Generating and identifying axolotls with targeted mutations using Cas9 RNA-guided nuclease / G. Parker Flowers and Craig M. Crews -- Gene manipulation for regenerative studies using the Iberian ribbed Newt, Pleurodeles waltl / Toshinori Hayashi and Takashi Takeuchi -- Transcriptomics using axolotls / S. Randal Voss, Antony Athippozhy, and M. Ryan Woodcock -- Sal-site : research resources for the Mexican axolotl / Nour W. Al Haj Baddar, M. Ryan Woodcock, Shivam Khatri, D. Kevin Kump, and S. Randal Voss -- Data mining in Newt-omics, the repository for omics data from the newt / Mario Looso and Thomas Braun.
• Publisher Details: New York, NY : Humana Press
• Publication Date: 2015
• Extent: 1 online resource (x, 357 pages), illustrations (some color)
• Subjects: 571.8/89; Life sciences; Regeneration (Biology) -- Laboratory manuals; Salamanders -- Laboratory manuals; Regeneration (Biology); Salamanders; Urodela; Animals, Genetically Modified; Models, Animal; Regeneration; Cytology; Science -- Life Sciences -- Biology -- Molecular Biology; Cellular biology (cytology); Electronic books; Laboratory manuals; Electronic books; Laboratory Manuals
• Languages: English
• ISBNs: 9781493924950; 1493924958; 9781493924943
• Related ISBNs: 149392494X; 9781493924943
• Notes: Note: Includes bibliographical references and index.; Note: Online resource; title from PDF title page (SpringerLink, viewed March 13, 2015).
• Access Rights: Legal Deposit; Only available on premises controlled by the deposit library and to one user at any one time; The Legal Deposit Libraries (Non-Print Works) Regulations (UK).
• Access Usage: Restricted: Printing from this resource is governed by The Legal Deposit Libraries (Non-Print Works) Regulations (UK) and UK copyright law currently in force.