G protein-coupled receptor screening assays : methods and protocols /: methods and protocols. ([2015])

• Record Type: Book
• Further Information: Note: Edited by Duarte Miguel F. Prazeres, Sofia Aires M. Martins.
• Editors: Prazeres, Duarte Miguel F; Martins, Sofia Aires M
• Contents: G-Protein Coupled Receptors: An Overview of Signaling Mechanisms and Screening Assays -- Time–Resolved FRET Strategy to Screen GPCR Ligands Library -- Homogeneous Fluorescence Anisotropy-Based Assay for Characterization of Ligand Binding Dynamics to GPCRs in Budded Baculoviruses: The Case of Cy3B-NDP-a-MSH Binding to MC4 Receptors -- Construction of Recombinant HEK293 Cell Lines for the Expression of the Neurotensin Receptor NTSR1 -- cAMP Assay for GPCR Ligand Characterization: Application of BacMam Expression System -- Ca2+ Mobilization Assays in GPCR Drug Discovery -- Using Constitutive Activity to Define Appropriate High Throughput Screening Assays for Orphan G Protein-Coupled Receptors -- Monitoring G-Protein Coupled Receptor Activation Using the Protein Fragment Complementation Technique Split TEV -- Quantifying GPCR Internalization: A Focus on the Kisspeptin Receptor -- GPCR Oligomerization Analysis by Means of BRET and dFRAP -- Use of ImageJ to Recover Information from Individual Cells in a G-Protein Coupled Receptor Assay -- Methods to Immobilize GPCR on the Surface of SPR Sensors -- Olfactory Receptor Screening Assay Using Nanovesicle-Immobilized Carbon Nanotube Transistor -- Label-Free Biosensor Assays in GPCR Screening -- Multi-Dimensional GPCR Profiling and Screening Using Impedance-Based Label-Free and Real-Time Assay -- Label-Free Functional Selectivity Assays -- Measurement of Surface-Mediated Ca++ Transients on the Single-Cell Level in a Microfluidic Lab-on-a-chip Environment -- Cell-Based Assays and Animal Models for GPCR Drug Screening -- Computer-Aided Design of GPCR Ligands.
• Publisher Details: New York : Humana Press Springer
• Publication Date: 2015
• Copyright Date: 2015
• Extent: 1 online resource (xii, 296 pages), illustrations (some color)
• Subjects: 572; Medicine; G proteins -- Receptors; Drug development; Toxicology; Proteomics; Drug development; G proteins -- Receptors; Science -- Life Sciences -- Biochemistry; Proteins; Medical -- Pharmacology; Pharmacology; Chemistry, Pharmaceutical; Technology, Pharmaceutical; Investigative Techniques; Receptors, Cell Surface; Drug Therapy; Pharmacology; Analytical, Diagnostic and Therapeutic Techniques and Equipment; Membrane Proteins; Chemistry; Therapeutics; Biological Science Disciplines; Natural Science Disciplines; Proteins; Amino Acids, Peptides, and Proteins; Disciplines and Occupations; Chemicals and Drugs; Molecular Targeted Therapy; Drug Discovery; High-Throughput Screening Assays; Receptors, G-Protein-Coupled; Electronic books; Electronic books; Electronic books
• Languages: English
• ISBNs: 9781493923366; 1493923366; 1493923358; 9781493923359
• Related ISBNs: 9781493923359
• Notes: Note: Includes bibliographical references and index.
• Access Rights: Legal Deposit; Only available on premises controlled by the deposit library and to one user at any one time; The Legal Deposit Libraries (Non-Print Works) Regulations (UK).
• Access Usage: Restricted: Printing from this resource is governed by The Legal Deposit Libraries (Non-Print Works) Regulations (UK) and UK copyright law currently in force.