Xenopus : methods and protocols /: methods and protocols. (2018)

• Record Type: Book
• Further Information: Note: Edited by Kris Vleminckx.
• Editors: Vleminckx, Kris
• Contents: Preface…Table of Contents…Contributing Authors…1. Husbandry, General Care, and Transportation of Xenopus laevis, and Xenopus tropicalisSean McNamara, Marcin Wlizla, and Marko E. Horb2. Generation and Care of Xenopus laevis and Xenopus tropcalis EmbryosMarcin Wlizla, Sean McNamara, and Marko E. Horb3. Methods for CRISPR/Cas9 Xenopus tropicalis Tissue-Specific Multiplex Genome EngineeringThomas Naert and Kris Vleminckx4. Targeted Genome Engineering in Xenopus using the Transcription activator-Like Effector Nuclease (TALEN) TechnologyTom Van Nieuwenhuysen and Kris Vleminckx5. Genotyping of CRISPR/Cas9 Genome Edited Xenopus tropicalisThomas Naert and Kris Vleminckx6. BATCH-GE: Analysis of NGS Data for Genome Editing AssessmentWouter Steyaert, Annekatrien Boel, Paul Coucke, and Andy Willaert7. A Simple Knock-In System for Xenopus via Microhomology-Mediated End Jointing RepairKen-ich T. Suzuki, Yuto Sakane, Miyuki Suzuki, and Takashi Yamamoto8. How to Generate Non-Mosaic CRISPR/Cas9 Mediated Knock-In and Mutations F0 Xenopus through the Host-Transfer TechniqueEmmanuel Tadjuidje and Sang-Wook Cha9. Targeted Electroporation in the CNS in Xenopus EmbryosHovy Ho-Wai Wong and Christine E. Holt10. Conditional Chemogenetic Ablation of Photoreceptor Cells in Xenopus retinaAlbert Chesneau, Odile Bronchain, and Muriel Perron11. Cancer Models in Xenopus tropicalis by CRISPR/Cas 9 Mediated Knockout of Tumor SuppressorsThomas Naert and Kris Vleminckx12. CRISPR/Cas9 F0 Screening of Congenital Heart Disease Genes in Xenopus tropicalisEngin Deniz, Emily K. Mis, Muara Lane, and Mustafa K. Khokha13. Quantitative Proteomics of Xenopus Embryos I, Sample PreparationMeera Gupta, Matthew Sonnett, Lillia Ryazanova, Marc Presler, and Martin Wühr14. Quantitative Proteomics for Xenopus Embryos II, Data AnalysisMatthew Sonnett, Meera Gupta, Thao Nguyen, and Martin Wühr15. Dye Electroporation and Imaging of Calcium Signaling in Xenopus Nervous SystemLukas Weiss, Thomas Offner, Thomas Hassenklöver, and Ivan Manzini16. X-FaCT: Xenopus-Fast Clearing TechniquePierre Affaticati, Sébastien Le Mével, Arnim Jenett, Laurie Rivière, Elodie Machado, Bilal B. Mughal, and Jean-Baptiste Fini17. Cell Cycle Analysis of the Embryonic Brain of Fluorescent Reporter Xenopus tropicalis by Flow CytometryRivka Noelanders and Kris Vleminckx18. Manipulating and Analyzing Cell Type Composition of the Xenopus Mucociliary EpidermisPeter Walentek19. Evaluating Blood Cell Populations in Xenopus using Flow Cytometry and Differential Counts by CytospinJacques Robert, Eva-Stina Edholm, and Francisco De Jesus Andino20. Isolation and Culture of Amphibian (Xenopus laevis) Sub-Capsular Liver and Bone Marrow CellsAmulya Yaparla and Leon Grayfer21. Isolation and Primary Culture Methods of Adult and Larval Myogenic Cells from Xenopus laevisKazi Taheruzzaman and Akio Nishikawa.
• Publisher Details: New York, NY : Humana Press
• Publication Date: 2018
• Extent: 1 online resource (xi, 302 pages), illustrations (some color)
• Subjects: 597.8/654; Medicine; Xenopus -- Laboratory manuals; Xenopus -- Genetics -- Laboratory manuals; Human genetics; Medical -- Genetics; Medical genetics; Electronic books
• Languages: English
• ISBNs: 9781493987849; 1493987844
• Related ISBNs: 9781493987832; 1493987836
• Notes: Note: Online resource; title from PDF title page (SpringerLink, viewed September 5, 2018).
• Access Rights: Legal Deposit; Only available on premises controlled by the deposit library and to one user at any one time; The Legal Deposit Libraries (Non-Print Works) Regulations (UK).
• Access Usage: Restricted: Printing from this resource is governed by The Legal Deposit Libraries (Non-Print Works) Regulations (UK) and UK copyright law currently in force.