The LINC complex : methods and protocols /: methods and protocols. (2018)

• Record Type: Book
• Further Information: Note: Edited by Gregg G. Gundersen, Howard J. Worman.
• Editors: Gundersen, Gregg G; Worman, Howard J
• Contents: Part 1 - Structural and Biochemical Analysis of LINC complexes 1. EM Tomography of LINC Complexes Marie-Christin Spindler, Frederick Helmprobst, Christian Stigloher and Ricardo Benavente 2. Recombinant Purification of the Periplasmic Portion of the LINC Complex Victor E. Cruz and Thomas U. Schwartz 3. Analysis of High Molecular Weight Isoforms of Nesprin-1 and Nesprin-2 with Vertical Agarose Gel Electrophoresis Chloe Potter and Didier Hodzic 4. Analysis of Nesprin-2 Interaction with its Binding Partners and Actin Susumu Antoku and Gregg G. Gundersen 5. Interactions of Nesprin-4-containing LINC Complexes in Outer Hair Cells Explored by BioID Brian Burke Part 2 – Mechanical Aspects of the LINC Complex 6. Using Nesprin Tension Sensors to Measure Force on the LINC Complex Paul T. Arsenovic and Daniel E. Conway 7. Analyzing Mechanotransduction through the LINC Complex in Isolated Nuclei Nejma Belaadi, Angelique Millon-Frémillon, Julien Aureille, and Christophe Guilluy 8. Direct Force Probe for Nuclear Mechanics Vincent J. Tocco, Srujana Neelam, Qiao Zhang, Richard B. Dickinson, and Tanmay P. Lele 9. Centrifugal Displacement of Nuclei in Adherent Cells to Study LINC Complex Dependent Mechanisms of Homeostatic Nuclear Positioning Ruijun Zhu and Gregg G. Gundersen 10. Assembly and Use of a Microfluidic Device to Study Cell Migration in Confined Environments Jeremy Keys, Aaron Windsor, and Jan Lammerding Part 3 - Analysis of the LINC Complex in Model Systems and Development 11. Investigating LINC Complex Protein Homo-oligomerization in the Nuclear Envelopes of Living Cells Using Fluorescence Fluctuation Spectroscopy Jared Hennen, Isaac Angert, Kwang-Ho Hur, G.W. Gant Luxton, and Joachim D. Mueller 12. Functional Analysis of the Yeast LINC Complex Using Fluctuation Spectroscopy and Super Resolution Imaging Jay R. Unruh, Brian D. Slaughter, and Sue L. Jaspersen 13. Genetic Analysis of Nuclear Migration and Anchorage to Study LINC Complexes During Development of Caenorhabditis elegans Heidi N. Fridolfsson, Leslie A. Herrera, James N. Brandt, Natalie E. Cain, Greg J. Hermann, and Daniel A. Starr 14. High-resolution Imaging Methods to Analyze LINC Complex Function During Drosophila Muscle Development Alexander L. Auld, Mary Ann Collins, Torrey R. Mandigo, and Eric S. Folker 15. Computational Methods for Studying the Plant Nucleus Axel Poulet, Xiao Zhou, Kentaro Tamura, Iris Meier, Christophe Tatout, Katja Graumann, and David E. Evans 16. Investigation of Nuclear Periphery Protein Interactions in Plants Using the Membrane Yeast Two-Hybrid (MbY2H) System Maxime Voisin, Emmanuel Vanrobays, and Christophe Tatout 17. Immunolabeling Protocols for Studying Meiosis in Plant Mutants Defective for Nuclear Envelope Components Javier Varas and Mónica Pradillo Part 4 - LINC Complex in Mammalian Tissue, Organs and Disease 18. Generation and Analysis of Striated Muscle Selective LINC Complex Protein Mutant Mice Matthew J Stroud, Xi Fang, Jennifer Veevers, and Ju Chen 19. An in vitro System to Measure the Positioning, Stiffness and Rupture of the Nucleus in Skeletal Muscle William Roman, Mafalda R. Pimentel, and Edgar R. Gomes 20. Functional Analysis of LINC Complexes in Skin Iakowos Karakesisoglou, Carmen Mroß, and Angelika A. Noegel 21. Detection of SUN1 Splicing Variants at the mRNA and Protein Levels in Cancer Ayaka Matsumoto, Nariaki Matsuura, and Miki Hieda 22. Next-generation Sequencing and Mutational Analysis: Implications for Genes Encoding LINC Complex Proteins Peter L. Nagy and Howard J. Worman.
• Publisher Details: New York, NY : Humana Press
• Publication Date: 2018
• Extent: 1 online resource (xiv, 338 pages), illustrations (some color)
• Subjects: 571.6/5; Life sciences; Nuclear matrix -- Laboratory manuals; Cytoskeleton -- Laboratory manuals; Cytology; Science -- Life Sciences -- Biology -- Molecular Biology; Cellular biology (cytology); Electronic books
• Languages: English
• ISBNs: 9781493986910; 1493986910
• Related ISBNs: 9781493986903; 1493986902
• Notes: Note: Includes bibliographical references and index.; Note: Online resource; title from PDF title page (SpringerLink, viewed August 27, 2018).
• Access Rights: Legal Deposit; Only available on premises controlled by the deposit library and to one user at any one time; The Legal Deposit Libraries (Non-Print Works) Regulations (UK).
• Access Usage: Restricted: Printing from this resource is governed by The Legal Deposit Libraries (Non-Print Works) Regulations (UK) and UK copyright law currently in force.