Mechanobiology in Health and Disease. (2018)

• Record Type: Book
• Further Information: Note: Edited by Stefaan W. Verbruggen.
• Editors: Verbruggen, Stefaan W
• Contents: Front Cover; Mechanobiology in Health and Disease; Copyright; Contents; Contributors; Foreword to mechanobiology in health and disease; Preface; Acknowledgments; Chapter 1: Techniques for studying mechanobiology; 1. Introduction to Mechanobiology; 2. Animal Models and Tissue Engineering to Study Mechanobiology; 2.1. Analysis of a Single Cell; 2.1.1. Force application techniques to analyze a single cell; 2.1.1.1. Optical tweezers; 2.1.1.2. Atomic force microscopy; 2.1.1.3. Micropipette aspiration; 2.2. Cellular Interactions With Their Local Environment 2.2.1. Techniques to analyze cellular tractions2.2.1.1. Traction force microscopy; 2.2.1.2. Micropillar arrays; 2.3. Bioreactors to Mimic the in vivo Environment; 2.3.1. Types of bioreactors; 2.3.2. Future of bioreactors; 2.4. Animal Loading Models; 2.4.1. Noninvasive extrinsic skeletal loading models; 2.4.1.1. Tibial four-point bend model; 2.4.1.2. Ulnar compression model; 2.4.2. Embryonic animal models with an altered mechanical environment; 2.4.2.1. In ovo immobilization; 2.4.2.2. Mammalian models; 2.4.2.3. Zebrafish models; 2.5. Fluorescent Proteins (FPs) and Imaging Techniques 2.5.1. FPs as markers in mechanobiology2.5.2. Imaging technologies using FPs; 2.5.2.1. Live cell imaging; 2.5.2.2. Fluorescent resonance energy transfer (FRET); 2.5.2.3. Fluorescent recovery after photobleaching (FRAP); 2.5.2.4. Confocal and two-photon microscopy; 3. Molecular and Genetic Techniques to Study Mechanobiology; 3.1. Analysis of mRNA Expression; 3.1.1. Microarray analysis; 3.1.2. Transcriptomics: Total RNA and mRNA sequencing; 3.1.3. Quantitative real time PCR; 3.1.4. In situ hybridization; 3.2. Analysis at the Protein Level; 3.2.1. Immunohistochemistry; 3.2.2. Western blotting 3.2.3. ELISA3.3. Techniques for Editing Gene Function and Altering the Mechanical Environment; 3.3.1. In vitro mutagenesis-Mice; 3.3.2. CRISPR; 3.3.3. In ovo/ex ovo manipulation-Chick; 4. Computational Techniques in Mechanobiology; 4.1. Computational Modeling; 4.1.1. Computational fluid dynamics; 4.1.2. FE analysis; 4.1.3. Multiscale and multiphysics modeling; 4.2. Image Analysis; 4.2.1. Digital image correlation; 4.2.2. Particle image velocimetry; 5. Future Perspectives; References; Chapter 2: Cell geometric control of nuclear dynamics and its implications; 1. Introduction 1.1. Physical Link Between Nucleus and Cytoskeleton1.2. Microrheology of the Nucleus; 1.3. Boundary Conditions; 2. Nuclear Translational Motion; 2.1. Image Processing and Computational Methods; 2.2. Geometric Control of Nuclear Translation; 2.3. Role of Cytoskeleton in Nuclear Translation; 3. Nuclear Rotational Motion; 3.1. Image Processing and Computational Methods; 3.2. Cell Geometric Regulation of Nuclear Rotation; 3.3. Role of Cytoskeleton in Nuclear Rotation; 3.4. Implications of Nuclear Rotation in Cellular Functions; 4. Nuclear Envelope Fluctuations
• Publisher Details: Place of publication not identified : Elsevier Ltd Academic Press
• Publication Date: 2018
• Extent: 1 online resource
• Subjects: 612; Medicine; Human physiology; Molecular biology; Proteins; Cell physiology; Atomic structure; Molecular structure; Spectrum analysis; MEDICAL / Physiology; SCIENCE / Life Sciences / Human Anatomy & Physiology; Electronic books
• Languages: English
• ISBNs: 9780128129531; 0128129530
• Related ISBNs: 9780128129524
• Notes: Note: Includes bibliographical references and index.; Note: Online resource; title from PDF file page (EBSCO, viewed August 14, 2018).
• Access Rights: Legal Deposit; Only available on premises controlled by the deposit library and to one user at any one time; The Legal Deposit Libraries (Non-Print Works) Regulations (UK).
• Access Usage: Restricted: Printing from this resource is governed by The Legal Deposit Libraries (Non-Print Works) Regulations (UK) and UK copyright law currently in force.