The use of mass spectrometry technology (MALDI-TOF) in clinical microbiology. (2018)

• Record Type: Book
• Further Information: Note: Edited by Fernando Cobo.
• Editors: Cobo Martínez, Fernando
• Contents: Front Cover; The Use of Mass Spectrometry Technology (MALDI-TOF) in Clinical Microbiology; Copyright Page; Contents; List of Contributors; Preface; 1. Proteomics: Technology and Applications; 1.1 Introduction; 1.2 Definition and Importance of the Proteomics; 1.3 Technical Methods in Proteomics; 1.3.1 Isolation and separation of proteins; 1.3.2 Analysis of the structure of the separated proteins; 1.3.3 Utilization of computer databases to identify the characterized proteins; 1.4 Proteomics' Role in the Clinical Laboratory; 1.5 Clinical Applications of Proteomics; 1.5.1 Microbiology 1.5.2 Biomarkers1.5.3 Immunoproteomics; 1.5.4 Vaccines and drugs; 1.6 Bioinformatics' Application to Proteomics; 1.7 Challenges of Proteomics; 1.8 Conclusions; References; 2. Basis of Mass Spectrometry: Technical Variants; 2.1 Overview; 2.2 MS: Definition and Basic Principles; 2.3 A Brief Excursion to the History of MS and Related Nobel Prizes; 2.4 Basic Components of a Mass Spectrometer; 2.5 Ionization Techniques in Biological MS; 2.6 Mass Analyzers; 2.7 Tandem MS; 2.8 Separation Techniques Hyphenated With MS; 2.9 MSI and Other Ways of Examining Biological Tissues 2.10 Mass Spectrum, Data Representation, and ManagementAcknowledgments; References; 3. MALDI-TOF Commercial Platforms for Bacterial Identification; 3.1 Introduction; 3.2 Commercial MALDI-TOF MS Systems; 3.3 MALDI Biotyper System; 3.4 VITEK MS System; 3.5 MALDI Biotyper vs VITEK MS Systems' Comparison; 3.6 Andromas; References; 4. Work Procedures in MALDI-TOF Technology; 4.1 Introduction; 4.2 Reagents and Equipment; 4.2.1 VITEK MS; 4.2.1.1 Reagents; 4.2.1.2 Supplies; 4.2.1.3 Equipment; 4.2.2 MALDI Biotyper system; 4.2.2.1 Reagents; 4.2.2.2 Supplies; 4.2.2.3 Equipment 4.3 Calibration and Quality Control4.3.1 VITEK MS; 4.3.1.1 Calibration; 4.3.1.2 Controls; 4.3.2 MALDI Biotyper CA system; 4.3.2.1 Calibration; 4.3.2.2 Controls; 4.4 Preanalytical Processing Methods for Protein Extraction; 4.4.1 Specimen collection; 4.4.2 MALDI Biotyper clinical application system extraction protocol; 4.4.3 VITEK MS extraction protocol; 4.4.4 Special sample preparation methods (research use only); 4.4.4.1 Aerobic actinomycetes; 4.4.4.1.1 Nocardia; 4.4.4.1.2 Mycobacteria; 4.4.4.2 Filamentous fungi; 4.5 Analytical Procedure; 4.5.1 MALDI Biotyper system; 4.5.1.1 Inoculation 4.5.1.2 Spectrum acquisition4.5.1.3 Identification; 4.5.2 VITEK MS; 4.5.2.1 Inoculation; 4.5.2.2 Spectrum acquisition; 4.5.2.3 Identification; 4.6 Cleaning MALDI-TOF MS Target Plates; References; 5. Indications, Interpretation of Results, Advantages, Disadvantages, and Limitations of MALDI-TOF; 5.1 Introduction; 5.2 Indications for the Use of MALDI-TOF in Clinical Microbiology; 5.3 Interpretation of Results; 5.4 Advantages and Disadvantages; 5.4.1 Advantages; 5.4.2 Disadvantages; 5.5 Limitations of MALDI-TOF Technique; 5.5.1 Typing; 5.5.2 Resistance and virulence factors
• Publisher Details: London : Academic Press
• Publication Date: 2018
• Extent: 1 online resource
• Subjects: 543.0873; Mass spectrometry; Diagnostic microbiology; SCIENCE / Chemistry / Analytic; Electronic books
• Languages: English
• ISBNs: 9780128144527; 0128144521
• Related ISBNs: 9780128144510; 0128144513
• Notes: Note: Online resource; title from PDF title page (EBSCO, viewed August 9, 2018)
• Access Rights: Legal Deposit; Only available on premises controlled by the deposit library and to one user at any one time; The Legal Deposit Libraries (Non-Print Works) Regulations (UK).
• Access Usage: Restricted: Printing from this resource is governed by The Legal Deposit Libraries (Non-Print Works) Regulations (UK) and UK copyright law currently in force.