Monoclonal Antibodies in Diagnostic Immunohistochemistry. (1988)

• Record Type: Book
• Further Information: Note: Editor, Mark Wick.
• Editors: Wick, Mark
• Contents: Cover; Half Title; Title Page; Copyright Page; Preface; Contents; Contributors; 1 Theoretical and Technical Considerations for the Use of Monoclonal Antibodies in Diagnostic Immunohistochemistry; PART I; THEORETICAL CONSIDERATIONS; Monoclonal Antibodies: The Concept; Molecular Variability of Immunoglobulins; Generation of Variability; Generation of Variability: Practical Issues; Why Bother with MoAb?; Consistency; Specificity; Nature of Cross-Reactivity; Problems Associated with Specificity; How to Make MoAb; Spontaneous Generation of MoA b; Hybridoma Technology; PART II TECHNICAL CONSIDERATIONSSpecimen Procurement and Processing; Specimen Size; Use of Frozen Sections; Touch-Imprint Preparations and Cytological Specimens; Fixed Specimens; Effects of Decalcifying Solutions on Immunoreactivity; Freeze-Dried Tissues; Microwave-Fixed Specimens; Clearing Agents for Paraffin-Embedded Tissues; Blocking Steps; Incubation with Primary Antibodies; Immunohistochemical Development Systems; Direct, Labeled Antibody Methods; Indirect, Unlabeled Antibody Methods; Haptenated Antibody-Antihapten (HAAH) Procedures; Antibody-Staphylococcal Protein A-PAP (ASPAPAP) Procedures Colloidal Metal-Antibody Complex ProceduresCombined Antibody-Enzyme Procedures; Chromogens; Postchromogenic Enhancement Methods; Counterstains; Troubleshooting; Lack of Staining in Both Positive Control Sections and Test Tissues Possibilities.; Positive Controls Stain Appropriately, but Test Tissue Does Not Possibilities.; Background Staining Is Unacceptably High Possibilities.; Need for Negative Controls; Workup of Newly Accessioned Antibodies; REFERENCES; 2 Monoclonal Antibodies to Cytokeratins in Diagnostic Immunocytochemistry; BIOLOGY OF CYTOKERATINS DIAGNOSTIC IMMUNOCYTOCHEMISTRY OF CYTOKERATINSDIFFERENTIAL DIAGNOSIS OF EPITHELIAL NEOPLASMS USING ANTICYTOKERATIN MONOCLONAL ANTIBODIES; COMPARISON OF CYTOKERATIN ANTIBODIES; CYTOKERATIN EXPRESSION IN NONEPITHELIAL NEOPLASMS: GERM CELL NEOPLASMS, SARCOMAS, AND NEUROECTODERMAL TUMORS; COEXPRESSION OF CYTOKERATIN WITH OTHER INTERMEDIATE FILAMENT PROTEINS; FIXATION, IMMUNOCYTOCHEMICAL TECHNIQUE, AND INTERPRETATION OF IMMUNOSTAINS; CONCLUSIONS; REFERENCES; 3 Monoclonal Antibodies to Vimentin; BIOCHEMICAL CHARACTERISTICS OF VIMENTIN AND RELATIONSHIP TO OTHER INTERMEDIATE FILAMENT PROTEINS SENSITIVITY AND SPECIFICITY DATATECHNICAL CONSIDERATIONS; PRACTICAL APPLICATIONS OF VIMENTIN ANTIBODIES IN THE DIAGNOSIS OF NEOPLASTIC DISEASE; Spindle Cell Neoplasms (Table 2); Tumors of Skin; Tumors of Soft Tissue; Other Neoplasms That May Exhibit a Prominent Spindle Cell Component; Undifferentiated Small Round Cell Tumors; Epithelioid and Pleomorphic Tumors; Regional Differential Diagnosis of Carcinomas; Primary Intracranial Tumors; PRACTICAL APPLICATIONS OF VIMENTIN ANTIBODIES IN NONNEOPLASTIC DISEASES; PITFALLS IN THE INTERPRETATION OF IMMUNOSTAINS TO VIMENTIN; REFERENCES
• Publisher Details: Boca Raton, FL : CRC Press
• Publication Date: 1988
• Extent: 1 online resource
• Subjects: 616.07/56; Allergy; Clinical immunology; Allergy; Clinical immunology; Electronic books
• Languages: English
• ISBNs: 9781466593497; 1466593490
• Access Rights: Legal Deposit; Only available on premises controlled by the deposit library and to one user at any one time; The Legal Deposit Libraries (Non-Print Works) Regulations (UK).
• Access Usage: Restricted: Printing from this resource is governed by The Legal Deposit Libraries (Non-Print Works) Regulations (UK) and UK copyright law currently in force.