High-Performance Liquid Chromatography of Peptides and Proteins : Separation, Analysis, and Conformation /: Separation, Analysis, and Conformation. (2017)
- Record Type:
- Book
- Title:
- High-Performance Liquid Chromatography of Peptides and Proteins : Separation, Analysis, and Conformation /: Separation, Analysis, and Conformation. (2017)
- Main Title:
- High-Performance Liquid Chromatography of Peptides and Proteins : Separation, Analysis, and Conformation
- Further Information:
- Note: Editors, Colin T. Mant, Robert S. Hodges.
- Editors:
- Mant, Colin T
Hodges, Robert S - Contents:
- Cover; Title Page; Copyright Page; Preface; Table of Contents; SECTION I: PRACTICAL ASPECTS OF HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY; Properties of Peptides/Proteins and Practical Implications; Standard Chromatographic Conditions for Size-Exclusion, Ion-Exchange, Reversed-Phase and Hydrophobic Interaction Chromatography; Preventive Maintenance and Troubleshooting LC Instrumentation; Tools and Techniques to Extend LC Column Lifetimes; Mobile Phase Preparation and Column Maintenance; HPLC Guard Columns; On-Line Derivatization of Silica Supports for Regeneration of Reversed-Phase Columns SECTION II: THEORETICAL ASPECTS OF HIGH-PERFORMANCE LIQUID CHROMATOGRAPHYHPLC Terminology: Practical and Theoretical; High-Performance Liquid Chromatography of Peptides and Proteins: General Principles and Basic Theory; High-Performance Liquid Chromatography of Peptides and Proteins: Quantitative Relationships for Isocratic and Gradient Elution of Peptides and Proteins; SECTION III: SIZE-EXCLUSION CHROMATOGRAPHY; Requirement for Peptide Standards to Monitor Ideal and Non-Ideal Behavior in Size- Exclusion Chromatography; High-Perfonnance Size-Exclusion Chromatography of Proteins Size-Exclusion Chromatography of ProteinsSize-Exclusion High-Performance Liquid Chromatography of Integral Membrane Proteins and the Effect of Detergents on Immunological Activity; A Reversed-Phase Trace Enrichment Technique for the Loading of Size-Exclusion HPLC Columns; SECTION IV: ION-EXCHANGE CHROMATOGRAPHY; The UseCover; Title Page; Copyright Page; Preface; Table of Contents; SECTION I: PRACTICAL ASPECTS OF HIGH-PERFORMANCE LIQUID CHROMATOGRAPHY; Properties of Peptides/Proteins and Practical Implications; Standard Chromatographic Conditions for Size-Exclusion, Ion-Exchange, Reversed-Phase and Hydrophobic Interaction Chromatography; Preventive Maintenance and Troubleshooting LC Instrumentation; Tools and Techniques to Extend LC Column Lifetimes; Mobile Phase Preparation and Column Maintenance; HPLC Guard Columns; On-Line Derivatization of Silica Supports for Regeneration of Reversed-Phase Columns SECTION II: THEORETICAL ASPECTS OF HIGH-PERFORMANCE LIQUID CHROMATOGRAPHYHPLC Terminology: Practical and Theoretical; High-Performance Liquid Chromatography of Peptides and Proteins: General Principles and Basic Theory; High-Performance Liquid Chromatography of Peptides and Proteins: Quantitative Relationships for Isocratic and Gradient Elution of Peptides and Proteins; SECTION III: SIZE-EXCLUSION CHROMATOGRAPHY; Requirement for Peptide Standards to Monitor Ideal and Non-Ideal Behavior in Size- Exclusion Chromatography; High-Perfonnance Size-Exclusion Chromatography of Proteins Size-Exclusion Chromatography of ProteinsSize-Exclusion High-Performance Liquid Chromatography of Integral Membrane Proteins and the Effect of Detergents on Immunological Activity; A Reversed-Phase Trace Enrichment Technique for the Loading of Size-Exclusion HPLC Columns; SECTION IV: ION-EXCHANGE CHROMATOGRAPHY; The Use of Peptide Standards for Monitoring Ideal and Non-Ideal Behavior in Cation-Exchange Chromatography; Ion-Exchange High-Performance Liquid Chromatography of Peptides; Use of Ion-Exchange Cartridges for Batch Extraction in the Purification of Pituitary Peptides High-Performance Ion-Exchange Chromatography of ProteinsHigh-Performance Ion-Exchange Chromatography of Proteins: Application to the Purification of Enzymes; Anion Exchange High-Performance Liquid Chromatography of Viral Proteins; Sample Preparation and Application of Different HPLC Methods for the Separation of Hydrophobic Membrane Proteins; Cation-Exchange High-Performance Liquid Chromatography: Group Separation of Acidic and Basic Proteins Using Volatile Solvents; Separation of Protein Isoforms: An Ion-Exchange HPLC Separation of Bovine and Cardiac Troponin Complexes Assessment of Steroid Receptor Polymorphism by High-Performance Ion-Exchange ChromatographySECTION V: REVERSED-PHASE CHROMATOGRAPHY; Reversed-Phase Packings for the Separation of Peptides and Proteins by Means of Gradient Elution High-Performance Liquid Chromatography; Commercially Available Columns and Packings for Reversed-Phase HPLC of Peptides and Proteins; Requirement for Peptide Standards to Monitor Colunm Performance and the Effect of Column Dimensions, Organic Modifiers, and Temperature in Reversed-Phase Chromatography … (more)
- Publisher Details:
- Boca Raton, FL : CRC Press
- Publication Date:
- 2017
- Extent:
- 1 online resource
- Subjects:
- 574.19245
Chromatography
Biochemistry
Analytical Chemistry
High performance liquid chromatography
Proteins -- Analysis
Peptides -- Analysis
SCIENCE / Life Sciences / Biology
High performance liquid chromatography
Peptides -- Analysis
Proteins -- Analysis
Electronic books
Electronic books - Languages:
- English
- ISBNs:
- 9780203751947
0203751949
9781351440752
1351440756
9781351440745
1351440748 - Related ISBNs:
- 084936549X
9780849365492 - Access Rights:
- Legal Deposit; Only available on premises controlled by the deposit library and to one user at any one time; The Legal Deposit Libraries (Non-Print Works) Regulations (UK).
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- Restricted: Printing from this resource is governed by The Legal Deposit Libraries (Non-Print Works) Regulations (UK) and UK copyright law currently in force.
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- Available online (eLD content is only available in our Reading Rooms) ↗
- Physical Locations:
- British Library HMNTS - ELD.DS.264282
- Ingest File:
- 01_174.xml