Topology and Physics of Circular DNA (1992). (2017)

• Record Type: Book
• Authors: Vologodskii, Alexander
• Contents: Cover; Title Page; Copyright Page; PREFACE; TABLE OF CONTENTS; INTRODUCTION; Chapter 1 Basic Physical Properties of DNA; I. DNA Structure; A. Single-Stranded DNA; B. The Double Helix; C. Alternative Forms of the Double Helix; D. Other Regular Structures; E. Hairpins and Cruciform Structures; II. Flexibility of the Double Helix; A. Macromolecular Conformation in Solution; B. Chains with Correlated Direction of Adjacent Links; C. Persistence Length of DNA; D. Excluded Volume Effect; E. Torsional Rigidity of the Double Helix; III. Conformational Transitions A. General Properties of Conformational TransitionsB. Statistical-Mechanical Treatment of Conformational Transition; C. Helix-Coil Transitions; D. B-A Transition; E. B-Z Transition; Chapter 2 Circular DNA and Supercoiling; I. The Circular Form of DNA; A. The Discovery of Circular D N A; B. The Topological Aspect of Inter-Strand Linkage; C. Linking Number Difference and Superhelical Density: Topoisomers; II. Experimental Studies of Supercoiling; A. Determining the Linking Number Difference; B. The Dependence of Superhelical Density on Solution Conditions C. Superhelicity of Circular DNA Isolated from CellsD. Obtaining DNA with a Preset Superhelical Density; III. Supercoiling Energy; IV. Topoisomerases; Chapter 3 Geometry and Topology of Circular DNA; I. Ribbon Theory; A. Writhe and Twist; B. Principal Properties of Writhe; II. Conformations of Supercoiled DNA; A. Torsional Rigidity of the Double Helix; B. Computer Simulation of Supercoiling; C. Experimental Studies of Supercoiled DNA Conformations; D. Thermodynamics of Supercoiling; III. Cyclization of DNA; IV. Knotted and Linked DNA; A. Types of Knots and Linkages B. Theoretical Analysis of Knots and LinkagesC. Knots and Linkages in DNA; D. Topological Approach to the Study of the Mechanisms of Enzymatic Reactions; Chapter 4 Formation of Noncanonical Structures under the Impact of Negative Supercoiling; I. Experimental Methods of Analysis of Noncanonical Structures in Circular DNA; A. Methods of Localization of Structural Transitions; B. The Method of Two-Dimensional Gel Electrophoresis; II. Thermodynamic Analysis of the Formation of Noncanonical Structures; III. Cruciform Structures; IV. The Left-Handed Z-Form; V. Melting of Closed Circular DNA VI. The H-FormVII. The Influence of Supercoiling on the B-A Transition; VIII. Statistical-Mechanical Analysis; IX. Mutual Influence and Competition of Different Structural Transitions; X. Kinetics of the Formation of Noncanonical Structures in Circular DNA; Chapter 5 The Use of Circular DNA in Studies of the Double Helix and Its General Properties; I. Finding the Helical Repeat of the Double Helix; II. Finding the Double Helix Unwinding Angle upon Ligand Binding; Chapter 6 DNA Supercoiling Inside the Cell; I. Superhelical Stress; II. The Existence of Noncanonical Structures within a Cell
• Publisher Details: Boca Raton, FL : CRC Press
• Publication Date: 2017
• Extent: 1 online resource
• Subjects: 574.87/3282; Biochemistry; Molecular Biology; Genetics; BIOSCIENCEnetBASE; CHEMLIBnetBASE; SCI-TECHnetBASE; CHEMISTRYnetBASE; LIFESCIENCEnetBASE; STMnetBASE; Circular DNA; SCIENCE / Life Sciences / Biology; Circular DNA; Electronic books; Electronic books
• Languages: English
• ISBNs: 9781315121307; 1315121301; 9781351355834; 9781351355827; 1351355821
• Related ISBNs: 135135583X; 1138105058; 9781138105058
• Access Rights: Legal Deposit; Only available on premises controlled by the deposit library and to one user at any one time; The Legal Deposit Libraries (Non-Print Works) Regulations (UK).
• Access Usage: Restricted: Printing from this resource is governed by The Legal Deposit Libraries (Non-Print Works) Regulations (UK) and UK copyright law currently in force.