Super-resolution microscopy : a practical guide /: a practical guide. (2017)

• Record Type: Book
• Further Information: Note: Udo J. Birk.
• Authors: Birk, Udo J
• Contents: Preface; Abbreviations; ; INTRODUCTION; The Classical Resolution Limit; Methods to Circumvent the Classical Resolution Barrier in Fluorescence Microscopy; Implementation of Super-Resolution Microscopy (SRM); Contrast; Applications to Study Nuclear DNA; Other Applications; ; PHYSICOCHEMICAL BACKGROUND; Motivation; Labeling; Transitions of the Fluorophores; Samples; ; HARD- AND SOFTWARE; Hardware Requirements; Software; Open Source and Best Practice; ; STRUCTURED ILLUMINATION AND IMAGE SCANNING MICROSCOPY; Axially Structured Illumination Microscopy (aSIM); Laterally Structured Illumination Microscopy (SIM); Image Scanning Microscopy; Super-Resolution Using Rotating Coherent Scattering (ROCS) Microscopy; ; LOCALIZATION MICROSCOPY; On the Principles of Localization Microscopy; PALM/STORM/fPALM/SPDM Approach; Implementation of SMLM; On the Principles of Three-Dimensional SMLM; Reduction of Out-of-Focus Light; How to Build a Three-Dimensional SMLM; High-Density Single Emitter Microscopy Methods: SOFI, 3B, SHRImP, etc; Approaches Towards Counting Molecules; Requirements and Sample Preparation; Data Acquisition; Data Analysis; Troubleshooting; Meta Analysis Tailored for SMLM; Example Applications; ; STIMULATED EMISSION DEPLETION MICROSCOPY (STED); On the Principles of Stimulated Emission Depletion Microscopy; Implementation of STED; Fluorescent Probes; Dye Combinations for Dual-Color STED; Requirements and Sample Preparation; Data Acquisition; Data Analysis and Visualization; Example Applications; Conclusion; ; MULTI-SCALE IMAGING; Light-Sheet Fluorescence Microscopy (LSFM); Optical Projection Tomography (OPT); Expansion Microscopy (ExM) and Sample Clearing; Alternative Approaches; ; ; DISCUSSION; Future Challenges; Commercialization of Super-Resolution Microscopes; Concluding Remarks; ; Index;
• Edition: 1st
• Publisher Details: Weinheim : Wiley-VCH
• Publication Date: 2017
• Extent: 1 online resource
• Subjects: 502.82; Microscopy -- Technique
• Languages: English
• ISBNs: 9783527802081; 9783527802050
• Related ISBNs: 9783527341337
• Notes: Note: Description based on CIP data; resource not viewed.
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