A structure-function toolbox for membrane transporter and channels. (2017)

• Record Type: Book
• Further Information: Note: Edited by Christine Ziegler.
• Editors: (Biochemist), Ziegler, Christine
• Contents: Title page; Table of Contents; Copyright; Contributors; Preface; Chapter One: Lipid Nanodiscs as a Tool for High-Resolution Structure Determination of Membrane Proteins by Single-Particle Cryo-EM; Abstract; 1 Introduction; 2 General Considerations; 3 Single-Particle Cryo-EM of the Ryanodine Receptor RyR1 Reconstituted Into Lipid Nanodiscs; 4 EM Analysis of the TcdA1 Pore Complex Embedded in Lipid Nanodiscs; 5 Conclusions and Perspectives; Acknowledgments; Chapter Two: SSM-Based Electrophysiology for Transporter Research; Abstract; 1 Background; 2 Technical Implications 3 Description of the Workflow4 Assay Development; 5 Data Interpretation and Analysis; Chapter Three: Saposin-Lipoprotein Scaffolds for Structure Determination of Membrane Transporters; Abstract; 1 Introduction; 2 Membrane Protein Reconstitution; 3 EM of Salipro Particles; 4 Summary and Conclusion; Acknowledgments; Chapter Four: Single-Molecule Fluorescence Studies of Membrane Transporters Using Total Internal Reflection Microscopy; Abstract; 1 Introduction; 2 Preparing Membrane Proteins and Lipid Membranes; 3 Single-Molecule Fluorescence Microscopy; 4 Data Analysis; Acknowledgments Chapter Five: The MEMbrane Protein Single ShoT Amplification Recipe: MemStarAbstract; 1 Introduction; 2 Materials; 3 Procedure; 4 Summary and Conclusion; Acknowledgments; Chapter Six: Efficient Screening and Optimization of Membrane Protein Production in Escherichia coli; Abstract; 1 Introduction; 2 Preparation of Expression Vectors and Cloning; 3 Analysis of Membrane Protein Production Using GFP; 4 Summary and Conclusion; Acknowledgments Chapter Seven: Pinning Down the Mechanism of Transport: Probing the Structure and Function of Transporters Using Cysteine Cross-Linking and Site-Specific LabelingAbstract; 1 Introduction; 2 The Unique and Useful Chemistry of Cysteine; 3 The Many Uses of Cysteine; 4 Cysteine-Reactive Reagents Used for Cross-Linking and Labeling; 5 Methods of Detecting Cross-Link Formation; 6 Strategies Used to Decide the Position of Cysteine Residues; 7 Detailed Protocol for Cross-Linking a Transporter Chapter Eight: Ion Channel Conformation and Oligomerization Assessment by Site-Directed Spin Labeling and Pulsed-EPRAbstract; 1 Introduction; 2 Designing the Pulsed-EPR Experiment; 3 Sample Preparation for EPR Experiments; 4 The EPR Methodology Toolbox; Acknowledgments; Chapter Nine: Strategy for the Thermostabilization of an Agonist-Bound GPCR Coupled to a G Protein; Abstract; 1 Introduction; 2 A Thermostability Assay for Measuring the Stability of GPCR-G Protein Complexes; 3 Summary and Conclusion; Acknowledgments; Author Index; Subject Index
• Publisher Details: Cambridge, MA : Academic Press is an imprint of Elsevier
• Publication Date: 2017
• Extent: 1 online resource
• Subjects: 571.6/4; Biological transport; Membrane proteins; Electronic books
• Languages: English
• ISBNs: 9780128123546; 0128123540
• Related ISBNs: 9780128123539; 0128123532
• Notes: Note: Online resource; title from PDF title page (ScienceDirect, viewed August 9, 2017).
• Access Rights: Legal Deposit; Only available on premises controlled by the deposit library and to one user at any one time; The Legal Deposit Libraries (Non-Print Works) Regulations (UK).
• Access Usage: Restricted: Printing from this resource is governed by The Legal Deposit Libraries (Non-Print Works) Regulations (UK) and UK copyright law currently in force.