Cannabinoids and their receptors. (2017)

• Record Type: Book
• Further Information: Note: Edited by Patricia H. Reggio.
• Other Names: Reggio, Patricia H
• Contents: 2.5. Reporter Assays of Downstream Signaling-Serum Response Element (SRE)2.5.1. Equipment; 2.5.2. Buffers and Reagents; 2.5.3. Procedure; 2.5.4. Notes; 2.6. Methods for Compound Suspension; 2.6.1. In Vitro Cellular Assays; 2.6.2. In Vivo Assays; 2.6.3. Notes; 3. Summary and Conclusions; References; Chapter Three: Real-Time Measurement of Cannabinoid Receptor-Mediated cAMP Signaling; 1. Introduction; 1.1. Canonical Cannabinoid Receptors Are GPCRs; 1.2. Importance of cAMP Signaling; 1.3. cAMP Signaling of Cannabinoid Receptors; 1.4. Temporal Control of GPCR Signaling 1.5. Types of cAMP Biosensor2. Detecting Cytoplasmic cAMP Using CAMYEL; 2.1. Protocol; 2.1.1. Equipment; 2.1.2. Cell Line Considerations; 2.1.3. Reagents; 2.1.4. Preparation of Cells-Seeding, Transfection, Reseeding of Adherent Cells; 2.1.5. Performing the Assay, Including Strategies for Seeing Gi vs Gs Signaling; 2.1.6. Cell Suspension Assay Variation; 2.2. Data Analysis; 3. Design and Validation of V8-CAMYEL; 3.1. Validation of V8-CAMYEL; 3.1.1. Stable Cell Lines; 4. Summary; References
• Publisher Details: Cambridge, MA : Elsevier Ltd Academic Press
• Publication Date: 2017
• Copyright Date: 2017
• Extent: 1 online resource
• Subjects: 615.7/827; Cannabinoids; Cannabinoids -- Receptors; Cannabinoids; Receptors, Cannabinoid; Cannabinoid Receptor Modulators; Cannabinoids -- therapeutic use; Electronic books
• Languages: English
• ISBNs: 9780128121573; 0128121572
• Related ISBNs: 9780128121566; 0128121564
• Notes: Note: Online resource; title from html information screen (ScienceDirect, viewed July 27, 2017).
• Access Rights: Legal Deposit; Only available on premises controlled by the deposit library and to one user at any one time; The Legal Deposit Libraries (Non-Print Works) Regulations (UK).
• Access Usage: Restricted: Printing from this resource is governed by The Legal Deposit Libraries (Non-Print Works) Regulations (UK) and UK copyright law currently in force.