Molecular morphology in human tissues : techniques and applications /: techniques and applications. (©2005)

• Record Type: Book
• Further Information: Note: Edited by Gerhard W. Hacker, Raymond R. Tubbs.
• Other Names: Hacker, Gerhard W; Tubbs, Raymond R, 1946-
• Contents: Cover Advances in Pathology, Microscopy, & Molecular Morphology MOLECULAR MORPHOLOGY in HUMAN TISSUES: Techniques and Applications DEDICATION FOREWORD PREFACE THE EDITORS ACKNOWLEDGMENTS CONTRIBUTORS CONTENTS 1 Immunostaining Techniques for Co -- Localization of Multiple Peptide Antigens in Light Microscopy 1.1 INTRODUCTION 1.2 SINGLE IMMUNOSTAINING ON CONSECUTIVE SECTIONS 1.3 DOUBLE OR TRIPLE IMMUNOSTAINING ON THE SAME SECTION 1.4 QUADRUPLE IMMUNOSTAINING ON THE SAME SECTION 1.5 CONCLUSIONS 1.6 PROTOCOLS I, II, III, IV, V, AND VI Acknowledgments References 2 Multiple Staining in Molecular Morphology 2.1 INTRODUCTION 2.2 DOUBLE STAINING CONCEPTS 2.3 MULTIPLE STAINING STRATEGY AND CONTROLS 2.4 SELECTING A CONCEPT, DETECTION SYSTEM, AND COLOR COMBINATION FOR DOUBLE STAINING 2.5 ADDITIONAL PRACTICAL TIPS AND COMMON MISTAKES WITH DOUBLE STAINING 2.6 PROTOCOLS AND TECHNICAL NOTES References ABBREVIATIONS 3 Enzyme-Based Fluorescence Amplification for Immunohistochemistry and In Situ Hybridization 3.1 INTRODUCTION 3.2 PROTOCOLS 3.3 RESULTS 3.4 TECHNICAL HINTS AND DISCUSSION Acknowledgments References 4 Gold Cluster Labels and Related Technologies in Molecular Morphology 4.1 INTRODUCTION: WHY CLUSTER LABELS? 4.2 CLUSTER LABELING METHODS AND RELATED TECHNOLOGIES 4.3 SPECIFIC GOLD CLUSTER PROBES AND METHODS References 5 Gold- and Silver-Facilitated Metallographic In Situ Hybridization Procedures for Detection of HER2 Gene Amplification 5.1 INTRODUCTION 5.2 FIXATION AND TISSUE PROCESSING 5.3 MATERIALS AND PROTOCOL PROCEDURE References 6 Toward Molecular Sensitivity: Tyramide Signal Amplification in Molecular Morphology 6.1 INTRODUCTION AND BRIEF HISTORICAL OVERVIEW 6.2 TYRAMIDE-AMPLIFIED NANOGOLD-SILVER/GOLD ISH 6.3 DISCUSSION 6.4 OUTLOOK Acknowledgments References 7 Array-Based Comparative Genomic Hybridization as a Tool for Survey of Genomic Alterations in Human Neoplasms 7.1 INTRODUCTION 7.2 MATERIALS AND PROTOCOLS 7.3 TROUBLESHOOTING References 8 Southwestern Histochemistry: A Method for Localization of Transcription Factors 8.1 INTRODUCTION 8.2 PROTOCOL A: FROZEN TISSUE SECTIONS 8.3 PROTOCOL B: PARAFFIN-EMBEDDED TISSUE SECTIONS 8.4 PROTOCOL C: CULTURED CELLS 8.5 RESULTS 8.6 TECHNICAL HINTS AND DISCUSSION Acknowledgments References 9 High Throughput Morphological Gene Expression Studies Using Automated mRNA In Situ Hybridization Applications and Tissue Microarrays for Post-Genomic and Clinical Research 9.1 INTRODUCTION 9.2 IMMUNOHISTOCHEMISTRY VS. ISOTOPIC MRNA ISH APPLICATIONS 9.3 AUTOMATED COLORIMETRIC MRNA ISH TECHNOLOGY 9.4 TISSUE MICROARRAYS 9.5 PROTOCOLS 9.6 RESULTS 9.7 TECHNICAL HINTS AND DISCUSSION 9.8 KEY FACTORS FOR SUCCESSFUL MRNA ISH EXPERIMENTS References 10 Whole-Mount In Situ Hybridization: Manual and Automated Procedure 10.1 INTRODUCTION 10.2 MATERIALS AND METHODS 10.3 TECHNICAL HINTS AND DISCUSSION References 11 An Open Door to Molecular Cytopathology: Interphase Fluorescence In Situ Hybridization (FISH) on Liquid-Based Thin-Layer Preparations 11.1 INTRODUCTION 11.2 TECHNICAL ASPECTS OF FISH 11.3 LIQUID-BASED THIN-LAYER PREPARATION AND ITS SUITABILITY FOR FISH 11.4 MATERIALS AND PROCEDURE 11.5 PITFALLS AND TROUBLESHOOTING 11.6 CONCLUSIONS References 12 Recent Developments in In Situ PCR 12.1 INTRODUCTION 12.2 REVIEW OF THE PCR TECHNIQUE 12.3 PRACTICAL PROTOCOLS References 13 High-Resolution Visualization of Apoptotic Markers in Human Biopsies 13.1 INTRODUCTION 13.2 MATERIAL AND REAGENTS 13.3 SECTIONING AND PREPARATION OF SLIDES 13.4 STAINING PROCEDURE 13.5 RESULTS 13.6 DISCUSSION References 14 Three-Dimensional Full Color Demonstration of Bright-Field and Fluorescence Microscopic Preparations: The Digital Optical Microscope 14.1 INTRODUCTION 14.2 METHODOLOGY 14.3 APPLICATIONS AND DISCUSSION 14.5 THE FUTURE References 15 Visual.
• Publisher Details: Boca Raton : CRC Press
• Publication Date: 2005
• Copyright Date: 2005
• Extent: 1 online resource (xxii, 304 pages), illustrations
• Subjects: 611/.018; Histochemistry -- Laboratory manuals; Histochemistry -- Laboratory manuals; MEDICAL -- Histology; Histochemistry; Histocytological Preparation Techniques; Immunohistochemistry -- methods; Humans; Electronic books; Laboratory manuals
• Languages: English
• ISBNs: 0203503481; 9780203503485
• Related ISBNs: 0849317029
• Notes: Note: Includes bibliographical references and index.; Note: Print version record.
• Access Rights: Legal Deposit; Only available on premises controlled by the deposit library and to one user at any one time; The Legal Deposit Libraries (Non-Print Works) Regulations (UK).
• Access Usage: Restricted: Printing from this resource is governed by The Legal Deposit Libraries (Non-Print Works) Regulations (UK) and UK copyright law currently in force.