Producing biomolecular substances with fermenters, bioreactors, and biomolecular synthesizers. (2006)

• Record Type: Book
• Further Information: Note: William L. Hochfeld.
• Other Names: Hochfeld, William L
• Contents: Introduction ; Biotechnology; Bioproduction; The Bioprocess; Cell Lines; Cell-Culture Process; Separation, Recovery, Purification; Virus and Foreign-DNA Removal; Quality Assurance; Technology Overview ; Biomolecular Foundations; Cellular Variation; Key Molecular Interactions; Base-Pair Complementarity; Genetic Coding; Interrupting Gene Segments; Sequence Determinations; Cloning; Synthesized DNA; Cell Lines; Expression Systems Introduction to the Bioprocess ; Overview; Optimizing Product Yield; Kinetic Models; cGMP Standards; Contract Manufacturing; Complex Mixtures; Biomolecular Products; Joint Manufacturing; Downscaling to Scale Up; Large-Scale Bioprocesses; Scaling-Up In-House or in a Contract Facility; Lab-to-Pilot-Plant-to-Production; Increasing Bioprocess Scale; Differing Features at Larger Scale; Simulating Environment at Scale; Detailed Specifications; Impact on Downstream Bioprocessing; FDA Biologics Review Process ; Regulating Biological Products Recombinant DNA Materials and Methods ; Cloning; Applications; Screening and Selection; DNA Synthesis; Cell Lines; Expression Systems; Vector Construction; Prokaryotic and Eukaryotic Growth Characteristics; Expression Levels; Intra- vs. Extra-cellular Expression; Intracellular Expression; Extracellular Expression; Glycosylation; Mammalian Cell Glycosylation; Insect Cell Glycosylation; General Considerations; Further Post-translational Modifications; Economic Concerns; Regulatory Concerns; Manufacturers’ Directory Enzymes ; Overview; Enzyme Protein Engineering; Transformation in Non-Aqueous Systems; Restriction Enzymes; Restriction-Enzyme Classes; Class I Restriction Enzymes; Class II Restriction Enzymes; Class III Restriction Enzymes; Restriction-Enzyme Specificity; Star Activity; Enzyme Families and Compatible Ends; Isoschizomers; Reverse Transcriptases; DNA and RNA Nucleases; Manufacturers’ Directory DNA-Amplification Reagents ; Overview; Amplification by DNA Synthesis (PCR); Amplification by RNA Transcription (TAS and 3SR; Amplification by Ligation (LAR, LCR, LAS); Amplification by RNA Replication (Q Replicase); Sequence-Based Amplification; Manufacturers Directory Cell-Culture Media ; Overview; Prokaryotic Cell-Culture Medium; Eukaryotic Cell-Culture Medium; Defined Medium; Serum; Other Sera; Serum Contamination; Serum-Free (Defined) Medium; Cell-Culture Gels; Media Supplements; Attachment Factors; Buffers; Sodium; Earle’s Balanced Salt Solution; Growth Factors; Epidermal Growth Factor (EGF); Fibroblast Growth Factors (FGF); Acidic FGF; Basic FGF; Other Growth Factors; Lectins; Toxins; Transport Factors; Vitamins; Water; Manufacturers’ Directory Fermenters ; Introduction to Fermentation; Historical Perspective; The Fermentation Process; Enzyme Fermentation in the Organic Phase; Cell Growth and Production; Factors Affecting Specific Growth Rate; Kinetic Models; Optimal Conditions; Recombinant Culture Kinetics; Fermenter Design; Kinetic Modeling of the Fermentation Process; STF Batch Scale Up; Power Number Method for STF Geometric Scale Up; Continuous-Culture Scale Up; Fermenter Manufacturers Directory Bioreactors ; Introduction; Aseptic Conditions; Monoclonal Antibody Production; mAB Production in Ascites Fluid; Bioreactor Engineering and Design; Bioreactors II: Kinetic Modeling and Bioreactor Dynamics and Bioreactor Design Program; Bioreactor Manufacturers Directory Biomolecular Synthesizers ; DNA Synthesizers; Manufacturers Directory; Technology Overview; Peptides by Solid-Phase Synthesis; Manufacturers Directory; Biotechnology Glossary ; References and Recommended Reading Index
• Publisher Details: Boca Raton, FL : CRC/Taylor & Francis
• Publication Date: 2006
• Extent: 1 online resource (383 pages), illustrations
• Subjects: 660.6/3; Biomolecules -- Synthesis; Bioreactors; Bioreactors; Biotechnology -- methods; Biomolecules; Bioreactors; SCIENCE -- Biotechnology; Biomolecules -- Synthesis; Bioreactors; Electronic books; Electronic books
• Languages: English
• ISBNs: 9781420021318; 1420021311; 1280516437; 9781280516436
• Related ISBNs: 0849322707; 9780849322709
• Notes: Note: Includes bibliographical references (pages 357-372) and index.; Note: Print version record.
• Access Rights: Legal Deposit; Only available on premises controlled by the deposit library and to one user at any one time; The Legal Deposit Libraries (Non-Print Works) Regulations (UK).
• Access Usage: Restricted: Printing from this resource is governed by The Legal Deposit Libraries (Non-Print Works) Regulations (UK) and UK copyright law currently in force.