Correlative light and electron microscopy III. ([2017])

• Record Type: Book
• Further Information: Note: Edited by Thomas Müller-Reichert [and] Paul Verkade.
• Editors: Müller-Reichert, Thomas, 1962-; Verkade, Paul
• Contents: Front Cover; Methods in Cell Biology; Series Editors; Methods in CellBiology: Correlative Light and Electron Microscopy III; Copyright; Contents; Contributors; Preface: The Key Lies in the Right Combination; 1 -- Millisecond time resolution correlative light and electron microscopy for dynamic cellular processes; INTRODUCTION; 1. METHODS; 1.1 Chlamydomonas reinhardtii Culture; 1.2 Imaging and Embedding Chamber Preparation; 1.3 Light Microscopy; 1.4 Sample Preparation for Electron Microscopy; 1.4.1 Chemical postfixation and embedding; 1.4.2 Thin sectioning 1.4.3 Staining of the sections and application of fiducial markers1.5 Electron Tomography; 1.6 Registration of Light and Electron Microscopy Images; 1.7 Data Analysis; 1.8 Preparation of Flagellar Cross Sections for Correlative Light and Electron Microscopy Analysis; 2. INSTRUMENTATION AND MATERIALS; 2.1 Chlamydomonas reinhardtii culture; 2.2 Imaging and Embedding Chamber Preparation; 2.3 Light Microscopy; 2.4 Sample Preparation for Electron Microscopy; 2.4.1 Chemical postfixation and embedding; 2.4.2 Thin sectioning; 2.4.3 Staining of the sections and application of fiducial markers 2.5 Electron Tomography2.6 Registration of Light and Electron Microscopy Images; 2.7 Data Analysis; 2.8 Preparation of Flagellar Cross Sections for Correlative Light and Electron Microscopy Analysis; 3. DISCUSSION AND OUTLOOK; Acknowledgments; REFERENCES; 2 -- 3D subcellular localization with superresolution array tomography on ultrathin sections of various species; INTRODUCTION AND RATIONALE; 1. METHODS-CORE PROTOCOL; 1.1 High-Pressure Freezing; 1.2 Freeze Substitution; 1.3 Embedding; 1.4 Ultramicrotomy; 1.5 Light Microscopy; 1.6 Contrasting and Carbon Coating 1.7 Scanning Electron Microscopy1.8 Image Processing and Correlation of Structured Illumination Microscopy and Scanning Electron Microscopy Images; 1.9 Alignment and 3D Reconstruction; 1.9.1 IMOD; 1.9.2 Fiji and AMIRA; 2. METHODS-MODEL-SPECIFIC ADAPTATIONS AND CONSIDERATIONS; 2.1 Caenorhabditis elegans; 2.2 Trypanosoma brucei; 2.3 Social Insects (Apis mellifera and Cataglyphis fortis); 3. INSTRUMENTATION AND MATERIALS; 3.1 High-Pressure Freezing; 3.1.1 General; 3.1.2 Caenorhabditis elegans; 3.1.3 Trypanosoma brucei; 3.1.4 Cataglyphis fortis and Apis mellifera; 3.2 Freeze Substitution 3.3 Embedding3.4 Ultramicrotomy; 3.5 Light Microscopy; 3.5.1 Primary Antibodies; 3.6 Contrasting and Carbon Coating; 3.7 Scanning Electron Microscopy; 3.8 Image Processing and Correlation of Structured Illumination Microscopy and Scanning Electron Microscopy Images; 3.9 Alignment and 3D Reconstruction; CONCLUSIONS; Acknowledgments; REFERENCES; 3 -- Preserving the photoswitching ability of standard fluorescent proteins for correlative in-resin super-resolutio ...; INTRODUCTION; 1. RATIONALE; 2. MATERIALS; 2.1 Instrumentation; 2.2 Materials; 2.3 Chemicals; 3. METHODS; 3.1 Reagent Preparation
• Publisher Details: Amsterdam, Netherlands Boston, Mass : Elsevier / Academic Press
• Publication Date: 2017
• Copyright Date: 2017
• Extent: 1 online resource (xvii, 352 pages), illustrations (some color)
• Subjects: 502.8/25; Electron microscopy -- Technique; SCIENCE -- Electron Microscopes & Microscopy; Electron microscopy -- Technique; SCIENCE / General; Electronic books
• Languages: English
• ISBNs: 9780128099766; 0128099763
• Related ISBNs: 9780128099759; 0128099755
• Notes: Note: Includes bibliographical references.; Note: Print version record.
• Access Rights: Legal Deposit; Only available on premises controlled by the deposit library and to one user at any one time; The Legal Deposit Libraries (Non-Print Works) Regulations (UK).
• Access Usage: Restricted: Printing from this resource is governed by The Legal Deposit Libraries (Non-Print Works) Regulations (UK) and UK copyright law currently in force.