Proteomics in biology. Part A (2017)

• Record Type: Book
• Further Information: Note: Edited by Arun K. Shukla.
• Editors: Shukla, Arun K
• Contents: Front Cover; Proteomics in Biology, Part A; Copyright; Contents; Contributors; Preface; Chapter One: An Easy and Fast Protocol for Affinity Bead-Based Protein Enrichment and Storage of Proteome Samples; 1. Introduction; 2. StrataClean Beads for Protein Enrichment; 2.1. A General Overview of the Method; 3. General Protocol for the Use of StrataClean Beads in Proteomics; 3.1. Priming and Washing of the Beads; 3.2. Affinity Binding of Proteins in Solution; 3.3. Elution of the Purified Proteins; 4. Application of StrataClean Beads in Protein Sample Preparation and Life Science Research 4.1. Highly Diluted Protein Solutions4.2. Optimization and Monitoring of Biotechnological Processes/Screening of Biological Samples; 4.3. Storage and Shipping of Protein Samples; 5. Protocol Variations; 5.1. Storage of Primed Beads; 5.2. Duration of Bead Incubation; 5.3. Use of Buffers Containing Urea; 5.4. Influence of the Salt Concentration; 6. Conclusions; References; Chapter Two: Filter-Aided Sample Preparation: The Versatile and Efficient Method for Proteomic Analysis; 1. Introduction; 2. The FASP Procedures; 2.1. FASP Methods Overview; 2.2. Selection of the Ultrafiltration Device 2.3. Limitations in Sample Size2.4. FASP Allows Consecutive Protein Digestion With Different Proteases; 2.5. Multipurpose Applications of FASP; 3. Filter-Aided Sample Preparation Protocols; 3.1. Preparation of Lysates from Tissues and Cells; 3.2. Determination of Total Protein in Lysates by WF-Assay Using Fluorescence Microtiter Plate Reader; 3.3. MED-FASP Protocol; 3.4. FASP Protocol; 3.5. TAPEG-FASP Protocol; 3.5.1. Synthesis of TAPEG (Optional); 3.5.2. Workflow; Acknowledgments; References; Chapter Three: An Overview of Advanced SILAC-Labeling Strategies for Quantitative Proteomics 1. Introduction2. Dual Labeling to Exclude Unlabeled Proteins; 2.1. Cell-Cell Contact Analyses; 2.2. Secretion Analyses; 2.3. Analyses of Postmitotic Neurons; 2.4. Use of Nondialyzed Serum; 3. Subsaturating Labeling; 3.1. Increasing Measurement Accuracy for Medium to Slow Turnover Proteins; 3.2. Quadruple Subsaturating Labeling to Separately Measure Decay andSynthesis After Cellular Stimulation; 3.3. Determining the Contribution of Extra- vs Intracellular Amino Acids toProtein Synthesis; 4. 5-Plexed SILAC Labeling; 5. Conclusion; References Chapter Four: Label-Free and Standard-Free Absolute Quantitative Proteomics Using the "Total Protein" and "Proteomic R ...1. Introduction; 2. TPA: For Determination of Protein Contents and Concentrations; 3. Cell Size and Protein Copy Numbers Can Be Assessed by the Proteomic Ruler; 4. Protein Concentrations and Copy Numbers Provide Different Layers of Information; 5. Consistency of TPA Values With Biochemical Data; 5.1. DNA and RNA Content; 5.2. Enzymatic Activities; 5.3. Subcellular Fractionation; 6. Normalization and Comparison of Datasets Using DJ-1/PARK7 Titer; 7. Limitations
• Publisher Details: Cambridge, MA : Academic Press is an imprint of Elsevier
• Publication Date: 2017
• Extent: 1 online resource
• Subjects: 572/.6; Proteomics; SCIENCE / Life Sciences / Biochemistry; Electronic books
• Languages: English
• ISBNs: 9780128105344; 0128105348; 9780128097434; 0128097434
• Related ISBNs: 9780128097427
• Notes: Note: Online resource; title from title details screen (ScienceDirect, viewed January 30, 2017).
• Access Rights: Legal Deposit; Only available on premises controlled by the deposit library and to one user at any one time; The Legal Deposit Libraries (Non-Print Works) Regulations (UK).
• Access Usage: Restricted: Printing from this resource is governed by The Legal Deposit Libraries (Non-Print Works) Regulations (UK) and UK copyright law currently in force.