Analysis of protein post-translational modifications by mass spectrometry. (2016)

• Record Type: Book
• Further Information: Note: Edited by John R. Griffiths, Richard D. Unwin.
• Authors: Griffiths, John R
• Editors: Griffiths, John R, 1964-; Unwin, Richard D
• Contents: List of contributors Preface Chapter 1: Introduction; Rebecca Pferdehirt, Florian Gnad, Jennie R. Lill 1.1. Post-translational modification of proteins 1.2. Global versus targeted analysis strategies 1.3. Mass spectrometric analysis methods for the detection of post translational modifications 1.4. The importance of bioinformatics References Chapter 2: Identification and Analysis of Protein Phosphorylation by Mass Spectrometry; Dean E. McNulty, Timothy W. Sikorski, Roland S. Annan 2.1. Introduction to Protein Phosphorylation 2.2. Analysis of Protein Phosphorylation by Mass Spectrometry 2.3. Global analysis of protein phosphorylation by mass spectrometry 2.4. Sample preparation and enrichment strategies for phosphoprotein analysis by mass spectrometry 2.5. Multidimensional separations for deep coverage of the phosphoproteome 2.6. Computational and bioinformatic tools for phosphoproteomics 2.7. Concluding Remarks References Chapter 3: Analysis of Protein Glycosylation by Mass Spectrometry; David J. Harvey 3.1. Introduction 3.2. General structures of carbohydrates 3.3. Isolation and purification of glycoproteins 3.4. Mass spectrometry of intact glycoproteins 3.5. Site analysis 3.6. Glycan release 3.7. Analysis of released glycans 3.8. Mass spectrometry of glycans 3.9. Computer interpretation of MS data 3.10. Total glycomics methods 3.11. Conclusions References Chapter 4: Protein Acetylation and Methylation; Caroline Evans 4.1. Overview of protein acetylation and methylation 4.2. Mass spectrometry behavior of modified peptides 4.3. Global analysis 4.4. Enrichment 4.5. Bioinformatics 4.6. Summary References Chapter 5: Tyrosine Nitration; Xianquan Zhan, Ying Long, Dominic M. Desiderio 5.1. Overview of tyrosine nitration 5.2. Mass spectrometry behavior of nitrated peptides 5.3. Global analysis of tyrosine nitration 5.4. Enrichment strategies 5.5. Concluding remarks Abbreviations Acknowledgements References Chapter 6: Mass Spectrometry Methods for the Analysis of Isopeptides Generated from Mammalian Protein Ubiquitination and Sumoylation; Navin Chicooree and Duncan L. Smith 6.1. Overview of SUMO and Ub 6.2. Mass Spectrometry Behaviour of Isopeptides 6.3. Enrichment and Global Analysis of Isopeptides 6.4. Concluding Remarks and Recommendations References Chapter 7: The Deimination of Arginine to Citrulline; Andrew J. Creese, Helen J. Cooper 7.1. Overview of arginine to citrulline conversion: Biological importance 7.2. Mass spectrometry-based proteomics 7.3. Liquid chromatography and mass spectrometry behavior of citrullinated peptides 7.4. Global analysis of citrullination 7.5. Enrichment strategies 7.6. Bioinformatics 7.7. Concluding remarks Acknowledgements References Chapter 8: Glycation of Proteins; Naila Rabbani and Paul J. Thornalley 8.1. Overview of protein glycation 8.2. Mass spectrometry behavior of glycated peptides 8.3. Global analysis of glycation 8.4. Enrichment strategies 8.5. Bioinformatics 8.6. Concluding remarks References Chapter 9: Biological Significance and Analysis of Tyrosine Sulfation; Éva Klement 1, Éva Hundyadi-Gulyás 1, Katalin F. Medzihradszky 9.1. Overview of protein sulfation 9.2. Mass spectrometry behavior of sulfated peptides 9.3. Enrichment strategies and global analysis of sulfation 9.4. Sulfation site predictions References Chapter 10: The Application of Mass Spectrometry for the Characterisation of Monoclonal Antibody-Based Therapeutics; Rosie Upton, Kamila J. Pacholarz, David Firth, Sian Estdale and Perdita E. Barran 10.1. Introduction 10.2. Antibody Structure 10.3. N-linked Glycosylation 10.4. Antibody-Drug Conjugates 10.5. Biosimilars 10.6. Sorption thermodynamics of heavy metals by ENMs 10.7. Factors influencing heavy metal sorption by ENMs 10.8. Summary and future perspectives References Index
• Edition: 1st
• Publisher Details: Hoboken, New Jersey : John Wiley & Sons, Inc
• Publication Date: 2016
• Extent: 1 online resource, illustrations
• Subjects: 572.645; Post-translational modification; Mass spectrometry
• Languages: English
• ISBNs: 9781119250890; 9781119250883
• Related ISBNs: 9781119045854
• Notes: Note: Description based on CIP data; item not viewed.
• Access Rights: Legal Deposit; Only available on premises controlled by the deposit library and to one user at any one time; The Legal Deposit Libraries (Non-Print Works) Regulations (UK).
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