Molecular themes in DNA replication. (©2009)

• Record Type: Book
• Further Information: Note: Edited by Lynne S. Cox.
• Other Names: Cox, Lynne S (Lynne Suzanne)
• Contents: Chapter 1. Introduction: conserved steps in eukaryotic DNA replication: Template -- Origins -- Origin recognition -- Pre-initiation -- Initiation -- Elongation -- Termination -- Chromatid cohesion and segregation. Chapter 2. AAA and ATPases in loading replication factors: ORC and loading of Cdc6 -- Cdc6 and loading of MCMs -- RFC and loading of PCNA. Chapter 3. Ring structures and six fold symmetry in replication: Helicases: MCMs -- WRN, BLM etc -- Clamps: PCNA -- Rad9-Rad1-Hus1. Chapter 4: Polymerization mechanisms: Thermodynamics of polymerization -- Structural insights into polymerization mechanisms -- fidelity: Replicative polymerase error rates -- Enhancing polymerase fidelity by protein interactions. Chapter 5. Molecular hand-off mechanisms in replication: Priming polymerization -- Polymerase switches -- Okazaki fragment processing: differential affinities for PCNA -- Methylation and chromatin modification via molecular hand-off mediated through PCNA? -- RPA in replication/repair. Chapter 6. Co-ordination of nucleases and helicases: Nucleases in replication: conservation of roles and mechanisms -- Fen1 and Dna2 at Okazaki fragments -- Stalled forks, helicases and nucleases -- BLM, WRN, Exo1 and others. Chapter 7. The switch from replication to repair (and back): Monitoring DNA integrity -- Stalled replication forks and replisome stability: impact of lesion type -- Choosing the right polymerase through PCNA modification -- Polymerase switching over lesions -- Restarting stalled forks after repair. Chapter 8: Telomeres and the end replication problem: Structure and stability of telomeres -- Telomerase: structural and drug inhibition studies -- ALT pathways of telomere maintenance -- Telomere length and telomere-specific sister chromatid exchange in replicative senescence. Keeping replicated chromatids together until mitosis: Topoisomerases and separating chromatids -- SMC proteins, claspins and cohesions -- Attaching chromatids to the mitotic spindle -- the role of ORC. Chapter 10. Chromatin modification during replication: Chromatin context and replication patterns -- Silenced loci and dormant origins -- DNA methylation during replication -- Histone modifications (methylation, acetylation, phosphorylation, ubiquitination -- CAF1, SIRT1, SNF2/HDACs etc) -- Nucleosome positioning in relation to origins. Chapter 11. Mittochondrial DNA replication: Template: copy number, structure and origin -- DNA pol gamma -- mode of action -- Accessory factors -- Regulation -- autonomous or integrated with cell cycle? -- Co-ordination with damage sensing and repair. Chapter 12. Archaeal paradigms and parallels for eukaryotic DNA replication: Overview: comparison between archaeal and eukaryotic replication proteins and processes -- Insights into priming from the structure of the primase core -- Archaeal MCMs as a model for eukaryotic MCMs -- Structure of the archaeal preRC and stabilization by GINS -- Protein interactions with PCNA. Chapter 13. Drug targets in DNA replication: Nuclear replication: ORC, MCMs and GINS -- markers or targets? -- Geminin -- PCNA (p21) and Ciz -- Polymerases -- Telomerase -- Mitochondrial replication targets -- Malaria and potential novel drug targets.
• Publisher Details: Cambridge : Royal Society of Chemistry
• Publication Date: 2009
• Copyright Date: 2009
• Extent: 1 online resource (xxi, 443 pages), illustrations (some color)
• Subjects: 572.8645; SCIENCE / Chemistry / Organic; DNA replication; Eukaryotic cells; SCIENCE -- Life Sciences -- Genetics & Genomics; DNA replication; Eukaryotic cells; SCIENCE / Life Sciences / Cell Biology; Cellular biology (cytology); Organic chemistry; Electronic books
• Languages: English
• ISBNs: 9781847559852; 1847559859
• Notes: Note: Includes bibliographical references and index.; Note: Online resource; title from title screen (viewed on June 8, 2010).
• Access Rights: Legal Deposit; Only available on premises controlled by the deposit library and to one user at any one time; The Legal Deposit Libraries (Non-Print Works) Regulations (UK).
• Access Usage: Restricted: Printing from this resource is governed by The Legal Deposit Libraries (Non-Print Works) Regulations (UK) and UK copyright law currently in force.