The zebrafish : genetics, genomics and informatics /: genetics, genomics and informatics. (2016)

• Record Type: Book
• Further Information: Note: Volume editors, H. William Detrich III, Leonard Zon, Monte Westerfield.
• Editors: III, Detrich, H. William; Westerfield, Monte; Zon, Leonard I
• Contents: Part 1. Forward and Reverse Genetics; 1. Multiplex Conditional Mutagenesis in Zebrafish Using the CRISPR/Cas System; 2. Tol2-Mediated Transgenesis, Gene Trapping and Enhancer Trapping, and the Gal4-UAS System; 3. Genetic Screens for Mutations Affecting Adult Traits and Parental-Effect Genes; 4. Targeted Candidate Gene Screens Using CRISPR/Cas9 Technology; 5. TALEN and CRISPR Enhanced DNA Homologous Recombination for Gene Editing in Zebrafish; 6. Precise Genome Editing by Homologous Recombination; 7. The Zebrafish Genome Editing Toolkit; 8. Clonal Analysis of Gene Loss-of-function and Tissue-specific Gene Deletion in Zebrafish via CRISPR/Cas9 Technology; 9. Tissue-Specific Gene Targeting Using CRISPR/Cas9; Part 2. Transgenesis and Functional Genomics; 10. Transcriptional Regulation Using the Q System in Transgenic Zebrafish; 11. Contemporary Zebrafish Transgenesis with Tol2 and Application for Cre/lox Recombination Experiments; 12. Method for Somatic Cell Nuclear Transfer in Zebrafish; 13. Experimental Approaches to Studying the Nature and Impact of Splicing Variation in Zebrafish; 14. PICCORO: A Technique for Manipulating the Activity of Transcription Factors with Blue Light; Part 3. Transcriptomics; 15. Tomo-seq: A Method to Obtain Genome-Wide Expression Data with Spatial Resolution; 16. Cell-Type Specific Transcriptomic Analysis by Thiouracil Tagging in Zebrafish; 17. RT-qPCR Gene Expression Analysis in Zebrafish: Preanalytical Precautions and Use of Expressed Repetitive Elements (ERE) for Normalization; Part 4. Epigenetics; 18. Genome-Wide DNA Methylation Profiling in Zebrafish; 19. Histone Modifications in Zebrafish Development; 20. Chromatin Immunoprecipitation and an Open Chromatin Assay in Zebrafish Erythrocytes; 21. Assay for Transposase-Accessible Chromatin and Circularized Chromosome Conformation Capture, Two Methods to Explore the Regulatory Landscapes of Genes in Zebrafish; 22. Epigenetic Regulation of Hematopoietic Stem Cell Development; Part 5. Zebrafish Infrastructure; 23. A Scientist’s Guide for Submitting Data to ZFIN; 24. New Frontiers for Zebrafish Management; 25. Aquaculture, Husbandry, and Shipping at the Zebrafish International Resource Center; 26. Health Monitoring and Disease Prevention at the Zebrafish International Resource Center
• Edition: Fourth edition
• Publisher Details: Amsterdam : Academic Press
• Publication Date: 2016
• Extent: 1 online resource
• Subjects: 597.482; Zebra danio; Zebra danio -- Research -- Methodology
• Languages: English
• ISBNs: 9780128034880
• Related ISBNs: 9780128034743
• Notes: Note: Description based on CIP data; item not viewed.
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