Preservation of cells : a practical manual /: a practical manual. (2016)

• Record Type: Book
• Further Information: Note: Al Hubel.
• Authors: Hubel, Al
• Contents: Proposed flow: the organization of the book follows the sequence of protocol development. Working table of contents 1. Introduction; a. Applications for preserved cells; b. Elements of preservation protocol; ; 2. Containers; a. Straws; b. Bags; c. Vials; d. Technical requirements; e. Closed system processing; ; 3. Pre-freeze processing; a. Influence of cell culture on freezing response; b. Liquid storage before cryopreservation; c. Selection of subpopulations and other pre-freeze processing processes; d. Evaluating the influence of pre-freeze processing on cell response; ; 4. Formulation of cryopreservation solutions; a. Cryoprotectants; b. Mechanisms of cryoprotection; c. Toxicity of cryoprotective agents; d. Development of introduction and removal protocols for cryopreservation solutions; e. Unique issues associated with formulation of solutions for cellular therapy products; ; 5. Freezing protocol; a. Importance of cooling rate; b. Controlled rate freezing protocols; i. Individual elements of the protocol; ii. Developing the elements of the protocols; c. Passive freezing; i. Development of a passive freezing protocol; ii. Validation of the protocol 6. Storage of frozen cells; a. Scientific basis for selection of a storage temperature; b. Review of scientific literature on storage of cells 7. Thawing; a. Methods of thawing; b. Critical thawing rate; c. Validating your thawing protocol; ; 8. Post thaw assessment; a. Different methods of post thaw assessment; b. Common problem with post thaw assessment; c. Post thaw apoptosis 9. Quality control and continuous assessment; ; Appendix: sample protocols
• Edition: 1st
• Publisher Details: Chichester, West Sussex : Wiley Blackwell
• Publication Date: 2016
• Extent: 1 online resource
• Subjects: 571.6; Cells -- Preservation
• Languages: English
• ISBNs: 9781118989876
• Notes: Note: Description based on CIP data; resource not viewed.
• Access Rights: Legal Deposit; Only available on premises controlled by the deposit library and to one user at any one time; The Legal Deposit Libraries (Non-Print Works) Regulations (UK).
• Access Usage: Restricted: Printing from this resource is governed by The Legal Deposit Libraries (Non-Print Works) Regulations (UK) and UK copyright law currently in force.