Intermediate filament proteins. (2016)

• Record Type: Book
• Further Information: Note: Edited by M. Bishr Omary and Ronald K.H. Liem.
• Editors: Omary, M. Bishr; Liem, Ronald Kian Hong, 1946-
• Contents: Front Cover; Intermediate Filament Proteins; Copyright; Dedication; Contents; Contributors; Preface; References; Acknowledgments; Part I: General Methods to Study IF Proteins; Chapter One: How to Study Intermediate Filaments in Atomic Detail; 1. Introductory Remarks; 2. Bioinformatics; 3. X-ray Crystallography; 3.1. Design of IF Protein Fragments for Crystallization; 3.2. Protein Expression; 3.3. Purification; 3.4. Crystallization; 3.5. X-ray Data Collection; 3.6. Phasing by Molecular Replacement; 3.7. Experimental Phasing Using Heavy Atoms; 3.8. Structure Refinement and Validation 3.9. Limitations and Pitfalls3.10. Obtained Structures and Impact; 4. Electron Paramagnetic Resonance with Site-Directed Spin Labeling; 4.1. Principle; 4.2. Sample Preparation and Measurements; 4.3. Data Interpretation and Impact; 4.4. Limitations and Outlook; 5. Concluding Remarks; Acknowledgments; References; Chapter Two: Mechanical Properties of Intermediate Filament Proteins; 1. Introduction; 2. Viscoelasticity of Purified IFs In Vitro; 3. IFs and the Mechanical Properties of Cells; 3.1. Type III IF: Vimentin and Desmin; 3.1.1. Knockout Models of Vimentin 3.1.2. Drugs and Proteins Disrupting Vimentin3.1.3. Mutated Desmin to Disrupt Vimentin; 3.1.4. Mutated Desmin to Disrupt Desmin; 3.2. Keratins; 3.3. Neurofilaments; 4. Conclusion; Acknowledgments; References; Chapter Three: Multidimensional Monitoring of Keratin Intermediate Filaments in Cultured Cells and Tissues; 1. Introduction; 2. 3D Imaging of Keratin Intermediate Filaments in Cultured Cells; 2.1. Microscopes; 2.2. Preparation of Imaging Medium; 2.3. Surface Coating; 2.4. Preparation of Cells for Imaging; 2.5. Image Acquisition; 2.6. Keratin Network Normalization 2.7. Measuring Keratin Movement2.8. Keratin Bulk Flow Analysis; 2.9. Keratin Turnover Measured by FRAP; 3. 3D Imaging of Keratin Intermediate Filaments in Murine Preimplantation Embryos; 3.1. Embryo Collection and Cultivation; 3.2. Imaging of Preimplantation Embryos; 3.3. Image Processing and Analysis; 4. Outlook; Acknowledgments; References; Chapter Four: Phospho-Specific Antibody Probes of Intermediate Filament Proteins; 1. Introduction; 2. Production of Site- and Phosphorylation State-Specific Antibodies; 2.1. Designing a Synthetic Phosphopeptide 2.2. Conjugation of a Phosphopeptide to a Carrier Protein3. Characterization of Site- and Phosphorylation State-Specific Antibodies; 3.1. ELISA; 3.2. Immunoblotting; 4. Immunocytochemistry; 4.1. Overview for Immunostaining Methods; 4.2. Vimentin Phosphorylation in Mitosis; 4.3. Vimentin Phosphorylation in Signal Transduction; 5. Other Applications; 6. Conclusions; Acknowledgments; References; Chapter Five: Assays for Posttranslational Modifications of Intermediate Filament Proteins; 1. Introduction; 1.1. Posttranslational Modifications of Intermediate Filament Proteins
• Publisher Details: Waltham, MA : Academic Press, an imprint of Elsevier
• Publication Date: 2016
• Copyright Date: 2016
• Extent: 1 online resource (xxv, 791 pages), illustrations (chiefly color)
• Subjects: 572/.67; Intermediate filament proteins; Intermediate Filament Proteins; SCIENCE -- Life Sciences -- Biochemistry; Intermediate filament proteins; Electronic books; Electronic books
• Languages: English
• ISBNs: 9780128034910; 0128034912
• Related ISBNs: 9780128034705; 012803470X
• Notes: Note: Includes bibliographical references and indexes.; Note: Online resource; title from HTML main page (ScienceDirect, viewed January 23, 2016).
• Access Rights: Legal Deposit; Only available on premises controlled by the deposit library and to one user at any one time; The Legal Deposit Libraries (Non-Print Works) Regulations (UK).
• Access Usage: Restricted: Printing from this resource is governed by The Legal Deposit Libraries (Non-Print Works) Regulations (UK) and UK copyright law currently in force.