Electrophoresis in practice : a guide to methods and applications of DNA and protein separations /: a guide to methods and applications of DNA and protein separations. (2016)

• Record Type: Book
• Further Information: Note: Reiner Westermeier.
• Authors: Westermeier, Reiner
• Contents: Contents; Part I; Fundamentals and History; Introduction; ; 1 Electrophoresis; ; 1.0 General; 1.1 Electrophoresis in non-restrictive gels; 1.1.1 Agarose gel electrophoresis; 1.1.2 Polyacrylamide gel electrophoresis of low-molecular weight substances; 1.2 Electrophoresis in restrictive gels; 1.2.1 The Ferguson plot; 1.2.2 Agarose gel electrophoresis; 1.2.3 Polyacrylamide gel electrophoresis of nucleic acids; 1.2.4 Polyacrylamide gel electrophoresis of proteins; 2 Isotachophoresis; 2.1 Migration with the same speed; 2.2 "Ion train" separation; 2.3 Zone sharpening effect; 2.4 Concentration regulation effect; 3 Isoelectric focusing; 3.1 Principles; 3.2 Gels for IEF; 3.3 Temperature; 3.4 Controlling the pH gradient; 3.5 The kinds of pH gradients; 3.5.1 Free carrier ampholytes; 3.5.2 Immobilized pH gradients; 3.6 Protein detection in IEF gels; 3.7 Preparative isoelectric focusing; 3.8 Titration curve analysis; ; 4 Blotting; 4.1 Principle; 4.2 Transfer methods; 4.3 Blotting membranes; 4.4 Buffers for electrophoretic transfers; 4.5 General staining; 4.6 Blocking; 4.7 Specific detection; 4.8 Protein sequencing; 4.9 Transfer problems; ; 5 Interpretation of electropherograms; 5.1 Introduction; 5.1.1 Purity control; 5.1.2 Quantification prerequisites; 5.2 Image analysis; 5.2.1 Hardware for image analysis; 5.2.2 Software for image analysis; ; 6 Proteome Analysis; 6.1 General; 6.2 Sample preparation; 6.3 Two-dimensional electrophoresis; 6.4 Detection techniques; 6.5 Image analysis; 6.6 Protein spot identification; 6.6.1 Mass spectrometry methods; 6.6.2 Peptide mass fingerprinting; 6.6.3 Protein characterization; 6.7 Bioinformatics; 6.8 Functional proteomics; ; 7 Instrumentation; 7.1 Current and voltage conditions; 7.2 Power supply; 7.3 Separation chambers; 7.3.1 Vertical apparatus; 7.3.2 Horizontal apparatus; 7.4 Staining apparatus for gels and blots; 7.5 Automated electrophoresis; 7.6 Instruments for 2-D electrophoresis; 7.6.1 Isoelectric focusing apparatus; 7.6.2 Multiple slab gel apparatus; 7.7 Safety measures; 7.8 Environmental aspects; ; Equipment for Part II; Instrumentation; Laboratory equipment; Consumables; Chemicals; ; Part II; Methods; Method 1: PAGE of dyes; 1 Sample preparation; 2 Stock solutions; 3 Preparing the casting cassette; 4 Casting the ultrathin-layer gels; 5 Electrophoretic separation; ; Method 2: PAGE of DNA Fragments; 1 Stock solutions; 2 Preparing the gels; 3 Sample preparation; 4 Electrophoresis; 5 Silver staining; ; Method 3: Agarose and immuno electrophoresis; 1 Sample preparation; 2 Stock solutions; 3 Preparing the gels; 4 Electrophoresis; 5 Protein detection; ; Method 4: Titration curve analysis; 1 Sample preparation; 2 Stock solutions; 3 Preparing the blank gels; 4 Titration curve analysis; 5 Coomassie and silver staining; 6 Interpreting the curves; ; Method 5: Native PAGE in amphoteric buffers; 1 Sample preparation; 2 Stock solutions; 3 Preparing the empty gels; 4 Electrophoresis; 5 Coomassie and silver staining; ; Method 6: Agarose IEF; 1 Sample preparation; 2 Preparing the agarose gel; 3 Isoelectric focusing; 5 Protein detection; ; Method 7: PAGIEF in rehydrated gels; 1 Sample preparation; 2 Stock solutions; 3 Preparing the blank gels; 4 Isoelectric focusing; 5 Coomassie and silver staining; 6 Perspectives; ; Method 8: Horizontal SDS-PAGE; 1 Sample preparation; 2 Stock solutions for the preparation of gels; 3 Preparing the casting cassette; 4 Gradient gel; 5 Electrophoresis; 6 Protein detection; 7 Blotting; 8 Perspectives; ; Method 9: Vertical PAGE; 1 Sample preparation; 2 Stock solutions; 3 Single gel casting; 4 Multiple gel casting; 5 Electrophoresis 210; 6 SDS electrophoresis of small peptides; 7 Two-dimensional electrophoresis; 8 DNA electrophoresis; 9 Long shelflife gels; 1
• Edition: Fifth edition
• Publisher Details: Weinheim : Wiley-VCH
• Publication Date: 2016
• Extent: 1 online resource, illustrations
• Subjects: 543.4; Electrophoresis; DNA
• Languages: English
• ISBNs: 9783527695195; 9783527695164; 9783527695171
• Related ISBNs: 9783527338801
• Notes: Note: Description based on CIP data; item not viewed.
• Access Rights: Legal Deposit; Only available on premises controlled by the deposit library and to one user at any one time; The Legal Deposit Libraries (Non-Print Works) Regulations (UK).
• Access Usage: Restricted: Printing from this resource is governed by The Legal Deposit Libraries (Non-Print Works) Regulations (UK) and UK copyright law currently in force.