Basic principles of analytical ultracentrifugation. (2015)

• Record Type: Book
• Further Information: Note: Peter Schuck, Huaying Zhao, Chad A. Brautigam, Rodolfo Ghirlando.
• Authors: Schuck, Peter; Zhao, Huaying; Brautigam, Chad A; Ghirlando, Rodolfo
• Contents: Analytical Ultracentrifugation Basics; PHYSICAL PRINCIPLES; DETECTION PRINCIPLES The Sedimenting Particle; PARTICLE-SOLVENT INTERACTIONS; PARTICLE-PARTICLE INTERACTIONS; SEDIMENTATION EFFECTS ON MACROMOLECULAR MIGRATION Sample Preparation and Ancillary Characterization ; COMMON SAMPLE REQUIREMENTS; CHOICE OF SOLVENT AND BUFFER; DETERMINING THE PARTIAL-SPECIFIC VOLUME Data Acquisition ; RADIAL DIMENSION; TEMPORAL DIMENSION; SIGNAL DIMENSION; SPECTRAL DIMENSION The Sedimentation Experiment; THE IMPORTANCE OF AVOIDING CONVECTION; ROTORS AND SAMPLE CELL ASSEMBLIES; TEMPERATURE; VACUUM; ROTOR SPEEDS; STARTING THE RUN; COMMENCING DATA ACQUISITION AND RUN DURATION; STOPPING THE RUN Control and Calibration Experiments ; DATA DIMENSIONS; STANDARD CONTROL EXPERIMENTS Appendix A: Macromolecular Partial-Specific Volumes; Appendix B: Solvent Properties; Appendix C: Refractive Index Increments; Appendix D: Solution Column
• Edition: 1st
• Publisher Details: Boca Raton : CRC Press
• Publication Date: 2015
• Extent: 1 online resource, illustrations (black and white, and colour)
• Subjects: 572; Ultracentrifugation
• Languages: English
• ISBNs: 9781498751162
• Related ISBNs: 9781498751155
• Notes: Note: Description based on CIP data; item not viewed.
• Access Rights: Legal Deposit; Only available on premises controlled by the deposit library and to one user at any one time; The Legal Deposit Libraries (Non-Print Works) Regulations (UK).
• Access Usage: Restricted: Printing from this resource is governed by The Legal Deposit Libraries (Non-Print Works) Regulations (UK) and UK copyright law currently in force.