Cryo-EM. Sample preparation and data collection / Part A, (©2010)

• Record Type: Book
• Further Information: Note: Edited by Grant J. Jensen.
• Other Names: Jensen, Grant J
• Contents: Part A: Sample preparation and data collection. 3d reconstruction from electron micrographs a personal account of its development -- Preparation of 2d crystals of membrane proteins for high-resolution electron crystallography data collection -- Helical crystallization of soluble and membrane binding proteins -- Plunge freezing for electron cryomicroscopy -- A practical guide to the use of monolayer purification and affinity grids -- Grafix: Stabilization of fragile macromolecular complexes for single particle cryo-EM -- Cryonegative staining of macromolecular assemblies -- Liposomes on a streptavidin crystal: A system to study membrane proteins by cryo-EM -- Micromanipulator-assisted vitreous cryosectioning and sample preparation by high-pressure freezing -- Site-specific biomolecule labeling with gold clusters -- How to operate a cryo-electron microscope -- Collecting electron crystallographic data of two-dimensional protein crystals -- Automated data collection for electron microscopic tomography -- Correlated light and electron cryo-microscopy -- Phase plates for transmission electron microscopy -- Radiation damage in electron cryomicroscopy.
• Publisher Details: San Diego, Calif : Academic Press/Elsevier
• Publication Date: 2010
• Copyright Date: 2010
• Extent: 1 online resource (xlv, 410 pages), illustrations
• Subjects: 502.825; Electron microscopy -- Technique; Proteins -- Structure; Genomics; Microscopy, Electron; SCIENCE -- Electron Microscopes & Microscopy; Electron microscopy -- Technique; Genomics; Proteins -- Structure; Electronic books
• Languages: English
• ISBNs: 9780080956954; 0080956955
• Related ISBNs: 9780123749062; 0123749069
• Notes: Note: Includes bibliographical references and indexes.
• Access Rights: Legal Deposit; Only available on premises controlled by the deposit library and to one user at any one time; The Legal Deposit Libraries (Non-Print Works) Regulations (UK).
• Access Usage: Restricted: Printing from this resource is governed by The Legal Deposit Libraries (Non-Print Works) Regulations (UK) and UK copyright law currently in force.