Microarray technology in practice. (2008)

• Record Type: Book
• Further Information: Note: Steven Russell, Lisa A. Meadows and Roslin R. Russell.
• Other Names: Russell, Steven (Steven R. H.); Meadows, Lisa Ann; Russell, Roslin R
• Contents: Contents; ; 1.What are microarrays?; A. The basics; 2.Designing and producing a microarray; A. Platform options; B. Oligonucleotide design; C. Whole genome or boutique arrays; D. The problems of multiple testing; E. Array layout; F. Quality assessment; ; 3.Sample collection and labelling; A. RNA extraction; B. Quality assessment; C. Direct labelling techniques; D. Indirect labelling techniques; E. End labelling techniques; F. Amplification techniques; G. Hybridization; ; 4.Data acquisition; A. Scanners; B. Image acquisition; C. Multiple scans and data merging; D. Finding spots; E. Background measurement; G. Quality control basics; ; 5.Experimental Design and Data Normalisation; A. Experimental design; B. Why normalize?; C. Unwanted signal variation; D. Common normalization approaches; E. Data transformation; F. Dealing with ‘S’-shaped trends; G. Replicates and spatial detrending; H. Normalization of single channel arrays; ; 6.Meta analysis; A. Data filtering; B. Screening for differentially expressed genes; C. Hierarchical clustering; D. Other clustering techniques; E. Components analysis; F. Prediction tools; ; 7.Data annotation, storage and submission; A. Functional data annotation; B. Microarray databases; C. Public repositories; ; 8.Applications in health & disease; A. Malaria - parasite expression; B. Cancer classification; C. Diagnostic Arrays; ; 9.It's not all gene expression; A. ChIP-array; B. CGH; C. Protein arrays; D. Cell arrays
• Publisher Details: Place of publication not identified : Academic Press
• Publication Date: 2008
• Extent: 1 online resource (464 pages)
• Subjects: 572.8636; DNA microarrays
• Languages: English
• ISBNs: 9780080919768; 0080919766
• Access Rights: Legal Deposit; Only available on premises controlled by the deposit library and to one user at any one time; The Legal Deposit Libraries (Non-Print Works) Regulations (UK).
• Access Usage: Restricted: Printing from this resource is governed by The Legal Deposit Libraries (Non-Print Works) Regulations (UK) and UK copyright law currently in force.