Immunology. (©2006)

• Record Type: Book
• Further Information: Note: Werner Luttmann [and three others].
• Other Names: Luttmann, Werner
• Contents: 1. Antibodies. Antibody characteristics -- Fabrication of antibodies -- Purification of antibodies -- Chemical coupling and marking of antibodies -- 2. Cell separation. Separation by cell size and cell density: centrifugation techniques -- Separation by means of cell-specific surface molecules -- 3. Flow cytometry. How does that work? -- Fluorescences -- Sample preparation -- Start-up of the flow cytometer -- Compensation and measuring -- Analysis -- Models and configurations -- 4. Quantitative immunoassays. Assay concepts -- Radioimmunoassay (RIA) -- Enzyme-linked immunosorbent assay (ELISA) -- ELISPOT assay -- Particle immunoassay (PIA) -- Amplification systems. 5. Western blot. Sample preparation -- Protein separation by gel electrophoresis -- Transfer of proteins to a membrane (Blot) -- Protein detection -- Dot and slot blot -- 6. In situ immunolocalization. Analyses of cell suspensions -- Tissue analysis -- Immunoelectron microscopic tissue analysis -- 7. Immunoprecipitation. the classics -- Immunoprecipitation "today" -- 8. The cell: live, eat, die. Cell viability -- Cell proliferation -- Phagocytosis assays -- Cell-mediated cytotoxicity -- Apoptosis assays -- 9. Special immunoassays. Determination of blood groups -- HLA typing -- Lymphoblast transformation -- 10. A short excursion into the unbeloved world of statistics. Descriptive statistics -- Inferential statistics.
• Publisher Details: Amsterdam Boston : Elsevier Academic Press
• Publication Date: 2006
• Copyright Date: 2006
• Extent: 1 online resource (xiv, 243 pages), illustrations
• Subjects: 616.07/9; Immunology; Immunologic Techniques; Immunologic Tests; MEDICAL -- Immunology; Immunology; Electronic books
• Languages: English
• ISBNs: 9780080916675; 0080916678
• Related ISBNs: 0120885441
• Notes: Note: Includes bibliographical references and index.; Note: Print version record.
• Access Rights: Legal Deposit; Only available on premises controlled by the deposit library and to one user at any one time; The Legal Deposit Libraries (Non-Print Works) Regulations (UK).
• Access Usage: Restricted: Printing from this resource is governed by The Legal Deposit Libraries (Non-Print Works) Regulations (UK) and UK copyright law currently in force.