Digital microscopy. (2007)

• Record Type: Book
• Uniform Title: Video microscopy.
• Further Information: Note: Edited by Greenfield Sluder, David E. Wolf.
• Other Names: Sluder, Greenfield; Wolf, D. E (David E.)
• Contents: Cover -- Contents -- Contributors -- Preface -- Chapter 1: Microscope Basics -- I. Introduction -- II. How Microscopes Work -- A. The Finite Tube-Length Microscope -- B. Infinity Optics Microscopes -- III. Objective Basics -- A. Types of Objectives -- B. "Mixing and Matching" Objectives -- C. Coverslip Selection -- IV. Mounting Video Cameras on the Microscope -- A. Basic Considerations -- B. Empty Magnification -- C. Camera Pixel Number and Resolution -- Reference -- Chapter 2: The Optics of Microscope Image Formation -- I. Introduction -- II. Physical Optics: The Superposition of Waves -- III. Huygens' Principle -- IV. Young's Experiment: Two-Slit Interference -- V. Diffraction from a Single Slit -- VI. The Airy Disk and the Issue of Microscope Resolution -- VII. Fourier or Reciprocal Space: The Concept of Spatial Frequencies -- VIII. Resolution of the Microscope -- IX. Resolution and Contrast -- X. Conclusions -- XI. Appendix I -- A. Fourier Series -- XII. Appendix II -- A. Kirchoff's Scalar Theory of Diffraction: Recasting Huygen's Principle in an Electrodynamic Context -- B. Generalizing the Problem -- C. Scalar Spherical Waves -- D. Green's Theorem -- E. Solution for a Plane -- F. Huygens' Principle -- XIII. Appendix III -- A. Diffraction by a Circular Aperture from Which the Airy Disk Comes -- Acknowledgments -- References -- Chapter 3: Proper Alignment of the Microscope -- I. Key Components of Every Light Microscope -- A. Light Source -- B. Lamp Collector -- C. Diffusers and Filters -- D. Field Diaphragm -- E. Condensers -- F. Aperture Diaphragm -- G. Condenser Carrier or Substage -- H. The Specimen Stage -- I. The Objective -- J. Revolving Nosepiece -- K. Infinity Space -- L. Tube, Eyepiece, and Video Adapters -- II. Koehler Illumination -- A. Aligning the Microscope for Koehler Illumination -- B. What Are the Benefits of Koehler Illumination? -- C. Resolution and Contrast -- Chapter 4: Mating Cameras to Microscopes -- I. Introduction -- II. Optical Considerations -- Chapter 5: Fundamentals of Fluorescence and Fluorescence Microscopy -- I. Introduction -- II. Light Absorption and Beer's Law -- III. Atomic Fluorescence -- IV. Organic Molecular Fluorescence -- V. Excited State Lifetime and Fluorescence Quantum Efficiency -- VI. Excited State Saturation -- VII. Nonradiative Decay Mechanisms -- VIII. Fluorescence Resonance Energy -- IX. Fluorescence Depolarization -- X. Measuring Fluorescence in the Steady State -- XI. Construction of a Monochromator -- XII. Construction of a Photomultiplier Tube -- XIII. Measuring Fluorescence in the Time-Domain -- A. Boxcar-Gated Detection Method -- B. Streak Camera Method -- C. Photon Correlation Method -- D. Note on the Process of "Deconvolution" -- E. Phase Modulation Method -- XIV. Filters for the Selection of Wavelength -- XV. The Fluorescence Microscope -- XVI. The Power of.
• Edition: 3rd ed
• Publisher Details: Amsterdam Boston : Elsevier Academic Press
• Publication Date: 2007
• Extent: 1 online resource (xvii, 608 pages, 9 pages of plates), illustrations (some color)
• Subjects: 570.282; Video microscopy; Image processing -- Digital techniques; Image processing -- Digital techniques; Video microscopy; Electronic books
• Languages: English
• ISBNs: 0080544347; 9780080544342
• Related ISBNs: 0123740258
• Notes: Note: Includes bibliographical references and index.; Note: Print version record.
• Access Rights: Legal Deposit; Only available on premises controlled by the deposit library and to one user at any one time; The Legal Deposit Libraries (Non-Print Works) Regulations (UK).
• Access Usage: Restricted: Printing from this resource is governed by The Legal Deposit Libraries (Non-Print Works) Regulations (UK) and UK copyright law currently in force.