Molecular protocols in transfusion medicine. (2000)

• Record Type: Book
• Further Information: Note: Gregory A. Denomme, Maria Rios, Marion E. Reid.
• Other Names: Denomme, Gregory A; Rios, Maria; Reid, Marion E
• Contents: Preface; ; List of Abbreviations; ; Part 1: Introduction; ; 1.1 Background; ; 1.2 Aim, Scope and Purpose of the Book; ; 1.3 Organization of the Book; ; 1.4 Terminology; ; 1.5 Regulatory Compliance; ; 1.6 References; ; Part 2: General Considerations; ; 2.1 Clinical Information; ; 2.2 Basic Principles of Molecular Analysis; ; 2.3 Troubleshooting; ; 2.4 References; ; Part 3: Protocols; ; 3.1 Sample Shipment and Storage; ; 3.2 Reagents; ; 3.3 Harvesting WBCs by RBC Sedimentation; ; 3.4 Short-term Cell Cultures from Amniotic Fluid; ; 3.5 Cultured Amniocyte Cell Harvesting; ; 3.6 Cell Preparation and DNA Extraction; ; 3.7 Assessment of DNA Quality, Quantity and Purity; ; 3.8 PCR Amplification; ; 3.9 Restriction Enzyme Digestion for PCR-RFLP Analysis; ; 3.10 Allele-specific PCR; ; 3.11 Agarose Gel Preparation and Electrophoresis; ; 3.12 Acrylamide Gel Preparation and Electrophoresis; ; 3.13 Single-strand Conformation Polymorphism (SSCP); ; 3.14 Optimizing a PCR; ; 3.15 Gene Zygosity Assessment by Quantitative PCR Amplification; ; 3.16 VNTR and STR Analysis by PCR Amplification; ; 3.17 Multiplex PCR Analysis; ; 3.18 Southern Analysis; ; 3.19 References; ; Part 4: Red Cell Blood Groups; ; 4.1 Terminology; ; 4.2 Clinical Applications; ; 4.3 Situations where Genotype and Phenotype do not Agree; ; 4.4 References; ; 4.5 Facts Sheets, Gene Maps and Molecular Protocols; ; Part 5: Platelet Blood Groups; ; 5.1 Terminology; ; 5.2 Clinical Applications; ; 5.3 References; ; 5.4 Facts Sheets, Gene Maps and Molecular Protocols; ; Part 6: Neutrophil Blood Groups; ; 6.1 Terminology; ; 6.2 Clinical Applications; ; 6.3 References; ; 6.4 Facts Sheets, Gene Maps and Molecular Protocols; ; Part 7: Appendices; ; 7.1 Database Information; ; 7.2 Glossary; ; 7.3 References; ; 7.4 Works Sheets and Request Forms; ; Index; ; Useful Tables;
• Publisher Details: Place of publication not identified : Academic Press
• Publication Date: 2000
• Extent: 1 online resource (186 pages)
• Subjects: 612.1/1825; 616.15; Blood groups -- Handbooks, manuals, etc; Genetic polymorphisms -- Handbooks, manuals, etc; Blood -- Transfusion -- Handbooks, manuals, etc; Blood Grouping and Crossmatching; Blood Transfusion; Polymorphism, Genetic; Hematology; Blood groups; Blood -- Transfusion; Genetic polymorphisms; Laboratory Manuals; Handbooks and manuals
• Languages: English
• ISBNs: 9780080536903; 0080536905
• Access Rights: Legal Deposit; Only available on premises controlled by the deposit library and to one user at any one time; The Legal Deposit Libraries (Non-Print Works) Regulations (UK).
• Access Usage: Restricted: Printing from this resource is governed by The Legal Deposit Libraries (Non-Print Works) Regulations (UK) and UK copyright law currently in force.