Biotechnology : A Laboratory Course /: A Laboratory Course. ([1996])

• Record Type: Book
• Further Information: Note: [edited by] Jeffrey M. Becker, Guy A. Caldwell, Eve Ann Zachgo.
• Editors: Becker, Jeffrey M; Caldwell, Guy A; Zachgo, Eve Ann
• Contents: Suggested Schedule for Laboratory Exercises; Introductory Notes; Record Keeping and Safety Rules; Format of Student Laboratory Records; The Ten Commandments of Record Keeping; Safety Rules in the Laboratory; Exercise 1: Aseptic Culture and Establishing Pure Cultures; Exercise 2: Preparation of Culture Media; Exercise 3: The Growth Curve; Exercise 4: Isolation of Plasmid DNA from Escherichia coli : The Mini-Prep; Exercise 5: Purification, Concentration, and Quantitation of DNA; Exercise 6: Large-Scale Isolation of Plasmid DNA by Column Chromatography; Exercise 7: Amplification of a lacZ ; Gene Fragment by the Polymerase Chain Reaction; Exercise 8: Restriction Digestion and Agarose Gel Electrophoresis; Exercise 9: Southern Transfer; Exercise 10: Preparation, Purification, and Hybridization of Probe; Exercise 11: Transformation of Saccharomyces cerevisiae ; Exercise 12: Isolation of Plasmid from Yeast and Escherichia coli Transformation; Exercise 13: Protein Assays; Exercise 14: Qualitative Assay for Galactosidase in Yeast Colonies; Exercise 15: Determination of Galactosidase in Permeabilized Yeast Cells; Exercise 16: Assay of Galactosidase in Cell Extracts; Exercise 17: Galactosidase Purification; Exercise 18: Western Blot: Probe of Protein Blot with Antibody to Galactosidase; Appendix 1: Alternate Protocols and Experiments; Exercise 1A: Isolation and Characterization of Auxotrophic Yeast Mutants; Exercise 2A: Measurement of pH; Exercise 3A: Use of the Spectrophotometer; Exercise 6A: Isolation of Plasmid DNA: The Maxi-Prep; Exercise 10A: Colony Hybridization; Appendix 2: Buffer Solutions; Appendix 3: Preparation of Buffers and Solutions; Appendix 4: Properties of Some Common Concentrated Acids and Bases; Appendix 5: Use of Micropipettors; Appendix 6: Safe Handling of Microorganisms. Appendix 7: List of Cultures; Appendix 8: Storage of Cultures and DNA; Appendix 9: Sterilization Methods; Appendix 10: Preparation of Stock Solutions for Culture Media; Appendix 11: Growth in Liquid Medium; Appendix 12: Determination of Viable Cells; Appendix 13: Determination of Cell Mass; Appendix 14: Determination of Cell Number; Appendix 15: Nomenclature of Strains; Appendix 16: Glassware and Plasticware; Appendix 17: Preparation of Tris and EDTA; Appendix 18: Basic Rules for Handling Enzymes; Appendix 19: Effects of Common Contaminants on Protein Assays; Appendix 20: Manufacturers' and Distributors' Addresses; Appendix 21: Surfing the Bionet: World Wide Web Addresses; Glossary; Subject Index.
• Edition: Second edition
• Publisher Details: San Diego : Academic Press
• Publication Date: 1996
• Extent: 1 online resource, illustrations
• Subjects: 660.6078; Biotechnology -- Laboratory manuals
• Languages: English
• ISBNs: 9780080528199; 0080528198
• Related ISBNs: 0120845628
• Notes: Note: Includes bibliographical references and index.
• Access Rights: Legal Deposit; Only available on premises controlled by the deposit library and to one user at any one time; The Legal Deposit Libraries (Non-Print Works) Regulations (UK).
• Access Usage: Restricted: Printing from this resource is governed by The Legal Deposit Libraries (Non-Print Works) Regulations (UK) and UK copyright law currently in force.