Enzyme kinetics and mechanism. Detection and characterization of enzyme reaction intermediates / Pt. F, (©2002)

• Record Type: Book
• Further Information: Note: Edited by Daniel L. Purich.
• Other Names: Purich, Daniel L
• Contents: ; Covalent Enzyme-Substrate Compounds: Detection and Catalytic Competence; Rapid Mix-Quench MALDI-TOF Mass Spectrometry for Analysis of Enzymatic Systems; Pre-Steady-State Kinetics of Enzymatic Reactions Studied by Electrospray Mass Spectrometry with On-Line Rapid-Mixing Techniques; Trapping of -Glycosidase Intermediates; Trapping Covalent Intermediates on ß-Glycosidases; 2-Hydroxy-6-keto-nona-2, 4-diene 1, 9-Dioic Acid 5, 6-Hydrolase: Evidence from18O Isotope Exchange for gem-Diol Intermediate; Nucleoside-Diphosphate Kinase: Structural and Kinetic Analysis of Reaction Pathway and Phosphohistidine Intermediate; Galactose-1-Phosphate Uridylyltransferase: Kinetics of Formation and Reaction of Uridylyl-Enzyme Intermediate in Wild-Type and Specifically Mutated Uridylyltransferases; Kinetic Evidence for Covalent Phosphoryl-Enzyme Intermediate in Phosphotransferase Activity of Human Red Cell Pyrimidine Nucleotidases; Characterization of (2->6) -Sialyltransferase Reaction Intermediates: Use of Alternative Substrates to Unmask Kinetic Isotope Effects; Use of Sodium Borohydride to Detect Acyl-Phosphate Linkages in Enzyme Reactions; Evidence for Phosphotransferases Phosphorylated on Aspartate Residue in N-Terminal DXDX(T/V) Motif; MurC and MurD Synthetases of Peptidoglycan Biosynthesis: Borohydride Trapping of Acyl-Phosphate Intermediates; Transaldolase B: Borohydride Reduction Trapping of Schiff Base Intermediate between Dihydroxyacetone and -Amino Group of Active-Site Lysine Residue; Use of NMR and Borohydride Trapping to Provide Evidence for Covalent Enzyme-Substrate Imine Intermediate; Detection of Covalent Tetrahedral Adducts by Differential Isotope Shift ¹³C NMR: Acetyl-Enzyme Reaction Intermediate Formed by 3-Hydroxy-3-methylglutaryl-CoA Synthase; Detection of Intermediates in Reactions Catalyzed by PLP-Dependent Enzymes: O-Acetylserine Sulfhydrylase and Serine-Glyoxalate Aminotransferase; Protein Tyrosine Phosphatase: X-Ray Crystallographic Observation of Cysteinyl-Phosphate Reaction Intermediate; GTP:GTP Guanylyltransferase: Trapping Procedures for Detecting and Characterizing Chemical Nature of Enzyme-Nucleotide Phosphoramidate Reaction Intermediate; -Glutamyl Thioester Intermediate in Glutaminase Reaction Catalyzed by Escherichia coli Asparagine Synthetase B; -Glutamyltranspeptidase and -Glutamyl Peptide Ligases: Fluorophosphonate and Phosphonodifluoromethyl Ketone Analogs as Probes of Tetrahedral Transition State and -Glutamyl-Phosphate Intermediate; Stoichiometric Redox Titrations of Complex Metalloenzymes; Urate Oxidase: Single-Turnover Stopped-Flow Techniques for Detecting Two Discrete Enzyme-Bound Intermediates; Nitric Oxide Synthase: Use of Stopped-Flow Spectroscopy and Rapid-Quench Methods in Single-Turnover Conditions to Examine Formation and Reactions of Heme- O2 Intermediate in Early Catalysis; Myeloperoxidase: Kinetic Evidence for Formation of Enzyme-Bound Chlorinating Intermediate; Time-Resolved Resonance Raman Spectroscopy of Intermediates in Cytochrome Oxidase; Porphobilinogen Deaminase: Accumulation and Detection of Tetrapyrrole Intermediates Using Enzyme Immobilization; Adenosylcobalamin-Dependent Glutamate Mutase: Pre-Steady-State Kinetic Methods for Investigating Reaction Mechanism; Ribonucleotide Reductase: Kinetic Methods for Demonstrating Radical Transfer Pathway in Protein R2 of Mouse Enzyme in Generation of Tyrosyl Free Radical; Galactose Oxidase: Probing Radical Mechanism with Ultrafast Radical Probe; Kinetic Characterization of Transient Free Radical Intermediates in Reaction of Lysine 2, 3-Aminomutase by EPR Lineshape Analysis; Demonstration of Peroxodiferric Intermediate in M-Ferritin Ferroxidase Reaction Using Rapid Freeze-Quench Mössbauer, Resonance Raman, and XAS Spectroscopies; A Survey of Covalent, Ionic, and Radical Intermediates in Enzyme-Catalyzed Reactions.
• Publisher Details: Amsterdam London : Academic Press
• Publication Date: 2002
• Copyright Date: 2002
• Extent: 1 online resource (xxxv, 515 pages), illustrations
• Subjects: 572.744; Enzyme kinetics; Enzymes -- Analysis; SCIENCE -- Life Sciences -- Biochemistry; Enzyme kinetics; Enzymes -- Analysis; Biofísica; Electronic books
• Languages: English
• ISBNs: 9780080489384; 0080489389
• Notes: Note: Includes bibliographical references and index.; Note: Print version record.
• Access Rights: Legal Deposit; Only available on premises controlled by the deposit library and to one user at any one time; The Legal Deposit Libraries (Non-Print Works) Regulations (UK).
• Access Usage: Restricted: Printing from this resource is governed by The Legal Deposit Libraries (Non-Print Works) Regulations (UK) and UK copyright law currently in force.