Methods in enzymology. Measuring biological responses with automated microscopy / Volume 414, ([2006])

• Record Type: Book
• Further Information: Note: Edited by James Inglese.
• Editors: Inglese, James
• Contents: Dynamic GFP Sensors for High-Content Analysis of the Cell Cycle; High Content Fluorescence-based Screening for Epigenetic Modulators; Development of Assays for Nuclear Receptor Modulators Using Fluorescently Tagged Proteins; The Ligand-Independent Translocation Assay: An Enabling Technology for Screening Orphan GPCRs by Arrestin Recruitment; High Content Screening of Known G Protein-Coupled Receptors by Arrestin Translocation; Cell Imaging Assays for G protein-coupled Receptor Internalization: Application to High Throughput Screening; High throughput confocal microscopy for beta arrestin-GFP translocation GPCR assays using the Evotec Opera; G-protein coupled receptor internalization assays in the High Content Screening Format; Screening for Activators of the Wnt/Fzd Pathway by Automated Fluorescent Microscopy; A live-cell, image based approach to understanding the enzymology and pharmacology of 2-bromopalmitate and palmitoylation; Quantitative single cell studies of nuclear receptor function using high-resolution HTM and image analyses; Tracking the motion of individual proteins with semiconductor quantum dots; Development and Application of Automatic High Resolution Light Microscopy for Cell Based Screens; Adenoviral Sensors for High-Content Cellular Analysis; Cell based assays in primary endothelial cells to study multiple steps in inflammation; Development and Implementation of Multiplexed Cell-Based Imaging Assays; High throughput screening for modulators of stem cell differentiation; High Content Kinetic Calcium Imaging in Drug Sensitive and Drug Resistant Human Breast Cancer Cells; Measurement and Analysis of Calcium Signaling in Heterogeneous Cell Cultures; Mulipliex Analysis of Inflammatory Signalling Pathways using a High Content Imaging System; Generation and Characterization of a Stable MK2-EGFP Cell Line and Subswquent Development of a High Content Imaging Assay on the Cellomics ArrayScan® Platform to Screen for p38 MAPK Inhibitors; Development and implementation of three MAPK signaling pathway imaging assays to provide MAPK module selectivity profiling for kinase inhibitors; MK2-EGFP translocation, c-Jun & ERK activation; Assay Development and Case History of a 32K Biased Library High Content MK2-EGFP Translocation Screen to Identify p38 MAPK Inhibitors on the ArrayScan® 3.1 Imaging Platform; Development and Implementation of a dual target MAPK high content assay (MK2-EGFP translocation & cJun activation) to provide LO support for kinase inhibitors; Compound classification using image-based cellular phenotypes; High Content Screening: Emerging Hardware and Software Technologies; An Infrastructure for High Throughput Microscopy: Instrumentation, Informatics, and Integration; Protein Translocation Assays: Key Tools for Accessing New Biological Information with High Throughput Microscopy; High-Content Screening of Functional Genomic Libraries; Fluorescent protein-based cellular assays analyzed by laser scanning microplate cytometry in 1536-well plate format; High-Throughput Measurements of Biochemical Responses Using the Plate::Vision™ Multimode 96 Mini-lens Array Reader; Systems Cell Biology Based on High Content Screening; Digital Autofocus Methods for Automated Microscopy; Fluorescence Lifetime Imagine Microscopy - Two-dimensional distribution measurement of fluorescence lifetime
• Publisher Details: San Diego, California : Academic Press is an imprint of Elsevier
• Publication Date: 2006
• Extent: 1 online resource (xliv, 691 pages), illustrations (some color)
• Subjects: 570.28/2; Fluorescence microscopy -- Technique; Enzymology -- Equipment and supplies; Microscopy, Fluorescence -- methods; Cell Physiological Phenomena; Automation; Cells; Microscopy, Fluorescence -- instrumentation; SCIENCE -- Microscopes & Microscopy; Electronic books
• Languages: English
• ISBNs: 9780080468983; 0080468985
• Notes: Note: Includes bibliographical references and indexes.; Note: Description based on online resource; title from digital title page (viewed on June 26, 2015).
• Access Rights: Legal Deposit; Only available on premises controlled by the deposit library and to one user at any one time; The Legal Deposit Libraries (Non-Print Works) Regulations (UK).
• Access Usage: Restricted: Printing from this resource is governed by The Legal Deposit Libraries (Non-Print Works) Regulations (UK) and UK copyright law currently in force.