Handbook of immunohistochemistry and in situ hybridization of human carcinomas. Molecular genetics, liver carcinoma and pancreatic carcinoma / Volume 3, (©2005)

• Record Type: Book
• Other Titles: Molecular genetics, liver carcinoma and pancreatic carcinoma
• Further Information: Note: Edited by M.A. Hayat.
• Other Names: Hayat, M. A, 1936-
• Contents: Contents; ; ; Authors and Coauthors of Volume 3; ; ; Foreword; ; ; Preface to Volume 3; ; ; Prologue; ; ; Selected Definitions; ; ; Classification Scheme of Human Cancers; ; ; I Molecular Genetics; ; ; 1 Quality Assurance in Immunohistochemistry; ; ; Introduction; ; ; Definitions; ; ; Principles of Quality Control in Immunohistochemistry; ; ; Technical Aspects of Individual Steps of Immunohistochemistry; ; ; References; ; ; 2 Methodology of Microarray Data Analysis; ; ; Introduction; ; ; Microarray Data Normalization and Transformation; ; ; Classification and Exploratory Data Analysis; ; ; Postanalysis Follow-Up and Validation of Microarray Data; ; ; Looking Ahead; ; ; References; ; ; 3 Microarray-Based Gene Expression Analysis of Defined Tumor Areas; ; ; Introduction; ; ; Materials and MethodS; ; ; Conclusion; ; ; References; ; ; 4 Tissue Microarray and Quantitative Fluorescence Image Analysis in Tumor Biomarker Analysis; ; ; Introduction; ; ; Materials; ; ; Methods; ; ; Results and Discussion; ; ; References; ; ; 5 Identification of Differentially Expressed Genes Using Rapid Subtraction Hybridization: Detailed Methodology for Performing Rapid Subtraction Hybridization; ; ; Introduction; ; ; Materials; ; ; Methods; ; ; Results and discussion; ; ; References; ; ; 6 Noncontact Laser Microdissection and Pressure Catapulting: A Basic Tool in Genomics, Transcriptomics, and Proteomics; ; ; The Force of Focused Light: An Introduction; ; ; History and Principle of Laser Cutting and Laser Pressure Catapulting; ; ; Sample Features and Specimen Preparation; ; ; Technical Setup of the PALM MicroBeam System; ; ; PALM RoboSoftware for Automated Microdissection and Catapulting; ; ; Automated Cell Recognition; ; ; RoboMover and RoboStage II; ; ; Applications of PALM MicroBeam: Overview and Outlook; ; ; References; ; ; 7 DNA Methylation Analysis of Human Cancer; ; ; Introduction; ; ; The Significance of DNA Methylation in Cancer; ; ; Clinical Significance of DNA Methylation in Tumors; ; ; Methods; ; ; Quantification of Global Methylation; ; ; Nonbisulfite Methods; ; ; Bisulfite Methods; ; ; Materials; ; ; Procedure; ; ; References; ; ; 8 Antiepidermal Growth Factor Receptor Antibody: Immunohistochemistry; ; ; Introduction; ; ; Materials and methods; ; ; Results and discussion; ; ; Conclusions; ; ; References; ; ; 9 PTEN and Cancer; ; ; Introduction; ; ; Materials and Methods; ; ; Materials; ; ; Methods; ; ; Materials; ; ; Methods; ; ; Results and Discussion; ; ; Conclusion; ; ; References; ; ; 10 The Role of p53 and p73 Genes in Tumor Formation; ; ; Gene Architecture of the p53 Family; ; ; TP63 and TP73 Play Important Roles in Development and Differentiation; ; ; Transcriptional and Apoptotic Activity of p63 and p73; ; ; Regulation of p73 and p63 Protein Stability and Transcriptional Activity; ; ; Role of p73 and p63 in Tumors; ; ; p73 Is Not a Classic Knudson-Type Tumor Suppressor; ; ; Alteration of p73 Expression in Human Cancer; ; ; Alteration of p63 Expression in Human Cancer; ; ; Upstream Components That Signal to p73 and p63; ; ; Mechanisms of Transdominance: Heterocomplex Formation and Promoter Competition; ; ; p73 and Chemosensitivity; ; ; Potential Application of p63/p73 in Gene Therapy of p53 Inactivated Tumors; ; ; Conclusions; ; ; References; ; ; 11 Analysis of Centrosome Amplification in Cancer; ; ; Introduction; ; ; Centrosome Structure; ; ; The Centrosome Cycle ; ; ; Coordination of the Centrosome, DNA, and Cell Cycles; ; ; The Centrosome and DNA Cycles Can Be Uncoupled; ; ; Evidence for a Centrosome-Based Cell Cycle Checkpoint; ; ; Centrosome Amplification in Cancer; ; ; Centrosome Amplification, Aneuploidy, and Chromosomal Instability; ; ; Methods for Analysis of Centrosome Amplification in Cancer; ; ; Sample Procurement; ; ; Morphological and Structural Methods; ; ; Functional Assay for Microtubule Nucleation and Growth; ; ; Conclusion; ; ; References; ; ; II Liver Carcinoma; ; ; 1 Liver Carcinoma; ; ; Hepatocellular Carcinoma; ; ; Genetics of Hepatocellular Carcinoma; ; ; Biomarkers for Hepatocellular Carcinoma; ; ; Aldose Reductase-like Protein; ; ; Beta-Catenin; ; ; Carcinoembryonic Antigen, CA19-9 and DU-PAN-2 Glycoproteins; ; ; CCND1; ; ; Cyclooxygenase-2; ; ; Glypican-3; ; ; Hepatitis B XAntigen; ; ; HER-2/neu; ; ; Inhibitor of Differentiation and DNA Binding Protein; ; ; LKB1 Gene; ; ; Metastatic Tumor Antigen1; ; ; Mitogen-Activated Protein Kinase; ; ; 17p; ; ; p53; ; ; PTEN; ; ; Smad4; ; ; Squamous Cell Carcinoma Antigen; ; ; Use of cDNA Microarray; ; ; Use of Proteomics; ; ; Role of Virus in Hepatocellular Carcinoma; ; ; Hepatitis B Virus; ; ; Hepatitis C Virus; ; ; Risk Factors; ; ; Treatment of Hepatocellular Carcinoma; ; ; Hepatoblastoma; ; ; References ; ; ; 2 Mechanisms of Human Hepatocarcinogenesis: An Overview; ; ; Introduction; ; ; Epidemiology of Hepatocellular Carcinoma; ; ; Natural History of Hepatocellular Carcinoma; ; ; Chronic Hepatitis; ; ; Cirrhosis; ; ; Phenotypic Alterations and Dysplasia; ; ;
• Publisher Details: Amsterdam Boston : Elsevier Academic Press
• Publication Date: 2005
• Copyright Date: 2005
• Extent: 1 online resource (1 volume), illustrations (chiefly color)
• Subjects: 572.8; 616.994/0756; Molecular genetics; Liver -- Cancer; Pancreas -- Cancer; SCIENCE -- Life Sciences -- Molecular Biology; Liver -- Cancer; Molecular genetics; Pancreas -- Cancer; Electronic books
• Languages: English
• ISBNs: 0080456251; 9780080456256
• Notes: Note: Includes bibliographical references and index.; Note: Print version record.
• Access Rights: Legal Deposit; Only available on premises controlled by the deposit library and to one user at any one time; The Legal Deposit Libraries (Non-Print Works) Regulations (UK).
• Access Usage: Restricted: Printing from this resource is governed by The Legal Deposit Libraries (Non-Print Works) Regulations (UK) and UK copyright law currently in force.