Chemokines. (2009)

• Record Type: Book
• Further Information: Note: Edited by Tracy M. Handel and Damon J. Hamel.
• Other Names: Handel, Tracy M; Hamel, Damon J
• Contents: Front Cover; Methods In Enzymology: Chemokines, Part A; Copyright Page; Contents; Contributors; Preface; Section 1: Chemokines and Receptors in Disease; Chapter 1: Chapter One Chemokines in Human Breast Tumor Cells: Modifying Their Expression Levels and Determining Their Effects ; 1. Introduction; 2. Modifying Chemokine Expression in Breast Tumor Cells; 3. Establishment of Primary Local Breast Tumors and Pulmonary Metastases; Acknowledgments; References; Chapter 2: Chapter 2 CCR5 Pharmacology Methodologies and Associated Applications; 1. Introduction. 2. CCR5 Signaling Assays and Application to Quantify and Characterize Ligand-Dependent Agonism, Antagonism, and Inverse Ag3. CCR5-Associated Ligand-Binding Assays; 4. Surrogate In Vitro Antiviral Assays; 5. CCR5 Site-Directed Mutagenesis and Ligand Docking Studies; 6. Non-HIV Indications-Associated Studies, Human CCR5 Knock-In Mice; References; Chapter 3: Chapter Three CXCR4 and Mobilization of Hematopoietic Precursors; 1. Introduction; 2. HSPC Mobilizing Agents that Target the CXCL12/CXCR4 Axis; 3. Donor Selection for HSPC Mobilization; 4. Flow Cytometric Enumeration of Mobilized HSPCs. 5. Dosing and Kinetics of HSPC Mobilization by G-CSF and Plerixafor6. Flow Cytometric Analysis of CXCR4 Expression on Human CD34+ Subsets; 7. Functional Characterization of Mobilized HSPCs; 8. Concluding Remarks; Acknowledgment; References; Chapter 4: Double-Label Nonradioactive ; 1. Introduction; 2. Basic Protocol for ISH (Using Digoxygenin-Labeled Probe); 3. Comments; References; Chapter 5: Chapter Five Expression of Chemokines and Chemokine Receptors in Human Colon Cancer; 1. Introduction; 2. Materials and Methods; 3. Results; 4. Discussion; Acknowledgments; References. Section II: Chemokine Related Proteins from PathogensChapter 6: Kaposi's Sarcoma Virally Encoded, G-Protein-Coupled Receptor: A Paradigm for Paracrine Transformation; 1. Introduction; 2. Cloning of vGPCR; 3. Assaying vGPCR Transforming Activity In Vitro; 4. vGPCR Transforming Activity In Vivo Using Xenograft Systems; 5. In Vivo Targeted Infection Using the TVA-RCAS System; 6. vGPCR-Induced Paracrine Transformation; 7. Characterization of vGPCR-Induced Molecular Signaling; 8. Evaluation of Activation of Second-Messenger-Generating Systems. 9. Activation of Signal-Transducing Protein Kinases and Small GTPases10. Akt Kinase Assay; 11. vGPCR Stimulated Activation of Rac1-Pulldown Assays; 12. Western Blotting Using Phospho-Specific Antibodies; 13. Activation of Transcription Factors; 14. Global Changes in Gene Expression: Microarray Analysis; 15. NFkappaB Luciferase Assays; 16. NFkappaB Binding Assays; 17. Nuclear Translocation of NFkappaB; Acknowledgments; References; Chapter 7: Chapter 7 Pharmacological and Biochemical Characterization of Human Cytomegalovirus-Encoded G Protein-Coupled Recept; 1. Introduction.
• Publisher Details: London San Diego, Calif : Academic Academic Press/Elsevier
• Publication Date: 2009
• Extent: 1 online resource (2 volumes), illustrations (color illustrations)
• Subjects: 616.079; Chemokines; Chemokines -- Physiological effect; Chemokines; Chemokines; Immune system; HEALTH & FITNESS -- Diseases -- General; MEDICAL -- Clinical Medicine; MEDICAL -- Diseases; MEDICAL -- Evidence-Based Medicine; MEDICAL -- Internal Medicine; Electronic books
• Languages: English
• ISBNs: 9780080956978; 0080956971; 9780080956961; 0080956963; 9780123749079; 0123749077; 9780128021958; 0128021950
• Related ISBNs: 9780123749086; 0123749085
• Notes: Note: Includes bibliographical references and index.; Note: Print version record.
• Access Rights: Legal Deposit; Only available on premises controlled by the deposit library and to one user at any one time; The Legal Deposit Libraries (Non-Print Works) Regulations (UK).
• Access Usage: Restricted: Printing from this resource is governed by The Legal Deposit Libraries (Non-Print Works) Regulations (UK) and UK copyright law currently in force.