Mitochondrial function. Mitochondrial electron transport complexes and reactive oxygen species / Part A, (2009)

• Record Type: Book
• Further Information: Note: Edited by William S. Allison and Immo E. Scheffler.
• Other Names: Allison, William S, 1935-; Scheffler, Immo E
• Contents: Front Cover ; Methods in Enzymology: Mitochondrial Function, Part A: Mitochondrial Electron Transport Complexes and Reactive Oxygen Species; Copyright Page; Contents; Contributors; Preface; Methods in Enzymology; Chapter 1: Visualizing Functional Flexibility by Three-Dimensional Electron Microscopy: Reconstructing Complex I of the Mitochondrial Respiratory Chain; 1. Introduction; 2. Random Conical Reconstruction; 3. Application of the Techniques to Complex I From Yarrowia Lipolytica; 4. Conclusion; Acknowledgments; References. Chapter 2: Correlated Light and Electron Microscopy/Electron Tomography of Mitochondria in Situ 1. Introduction; 2. Light Microscopy; 3. Electron Microscope Tomography; Acknowledgments; References; Chapter 3: Studies of Complex I by Fourier Transform Infrared Spectroscopy; 1. Spectroscopic Studies of Complex I; 2. FTIR Difference Spectroscopy and Its Application to Complex I; 3. Preparation of Rehydrated Layers of Complex I for ATR-FTIR Spectroscopy; 4. Analysis of Rehydrated Complex I Layers by Perfusion-Induced ATR-FTIR Difference Spectroscopy. 5. Analysis of Rehydrated Complex I Layers by Electrochemically-Induced ATR-FTIR Difference Spectroscopy6. Model Compounds and Strategies for Interpretation; 7. Example IR Difference Spectra of Complex I; Acknowledgments; References; Chapter 4: Electron Transfer in Respiratory Complexes Resolved by an Ultra-Fast Freeze-Quench Approach; 1. Introduction; 2. Ultra-Fast Freeze-Quench Setup; 3. Sample Preparation; 4. Calibration of the Freeze-Quench Setup; 5. Application of Freeze-Quench Approach for the Monitoring of Complex I Reduction by NADH; References. Chapter 5: Use of Ruthenium Photooxidation Techniques to Study Electron Transfer in the Cytochrome bc1 Complex1. Introduction; 2. Design and Synthesis of Ruthenium-Labeled Proteins; 3. Measurement of Interprotein Electron Transfer in a Ruthenium-Labeled Protein; 4. Measurement of Electron Transfer between Ruz-39-Cc and Cytochrome bc1; 5. Measurement of Electron Transfer within the Cytochrome bc1 Complex; Acknowledgments; References; Chapter 6: Mass Spectrometric Characterization of the Thirteen Subunits of Bovine Respiratory Complexes that are Encoded in Mitochondrial DNA; 1. Introduction. 2. Analysis of Mitochondrial Membranes for mt-Proteins3. Analysis of Respiratory Complexes for mt-Proteins; 4. Mass Spectrometric Analysis of mt-Proteins; 5. Conclusions and Perspectives; References; Chapter 7: Tracing Human Mitochondrial Complex I Assembly by Use of GFP-Tagged Subunits; 1. Introduction; 2. Selection of Complex I Subunit for GFP Labeling; 3. Creation of Inducible Cell Line Expressing AcGFP1-tagged NDUFS3; 4. Tracing AcGFP1-Labeled NDUFS3 on BN-PAGE; Acknowledgments; References.
• Publisher Details: Amsterdam Boston : Academic Press/Elsevier
• Publication Date: 2009
• Extent: 1 online resource (li, 537 pages, [16] pages of plates), illustrations
• Subjects: 571.6/57; Mitochondria; Active oxygen; Electron transport; Mitochondria; Reactive Oxygen Species; Electron Transport; Active oxygen; Electron transport; Mitochondria; Phosphoprotein phosphatases; Protein kinases; SCIENCE -- Life Sciences -- Cell Biology; Active oxygen; Electron transport; Mitochondria; Electronic books
• Languages: English
• ISBNs: 9780080915333; 0080915337; 9780080877761; 0080877761
• Notes: Note: Includes bibliographical references and index.; Note: Print version record.
• Access Rights: Legal Deposit; Only available on premises controlled by the deposit library and to one user at any one time; The Legal Deposit Libraries (Non-Print Works) Regulations (UK).
• Access Usage: Restricted: Printing from this resource is governed by The Legal Deposit Libraries (Non-Print Works) Regulations (UK) and UK copyright law currently in force.