Introduction to experimental biophysics : a laboratory guide /: a laboratory guide. (2015)

• Record Type: Book
• Further Information: Note: Jay L. Nadeau.
• Authors: Nadeau, Jay L
• Contents: Molecular Cloning; Transformation of Competent Bacteria with a DNA Plasmid; Isolation of Plasmids by Miniprep and Restriction Enzyme Analysis; Designing a New Construct Using Restriction Enzymes (Computer Lab); Restriction Enzymes; Ligation; Screening a Ligation and Choosing Recombinants; Use of Software to Design Primers for Cloning Using the Polymerase Chain Reaction (Computer Lab); Running the Polymerase Chain Reaction Gene Expression; Protein Expression and Purification; Characterization of Purified Protein; Protein Crystallization Fluorescent Reporter Genes in Mammalian Cells; Transfecting Mammalian Cells with Custom-Designed Constructs; Screening Fluorescent Reporter Gene Expression in Mammalian Cells Nanoparticles; Synthesis of Quantum Dots; Synthesis of Gold Nanoparticles Electrophysiology; Electrophysiology: Lipid Bilayer Recording; Electrophysiology Alternative: Introduction to Lipid Vesicles Appendices: Instructor Information and Guides; Appendix 1 Disposables and Supplies Needed; Appendix 2 Instructors’ Guide to Behind-the-Scenes Preparation; Appendix 3 Grading Recommendations and Guidelines; Appendix 4 Advanced Options: Customizing the Course Index
• Edition: 1st
• Publisher Details: Boca Raton : CRC Press
• Publication Date: 2015
• Extent: 1 online resource, illustrations (black and white)
• Subjects: 612.014; Biophysics -- Laboratory manuals
• Languages: English
• ISBNs: 9781466557666; 9781466557673
• Related ISBNs: 9781466557659
• Notes: Note: Includes bibliographical references and index.; Note: Description based on CIP data; item not viewed.
• Access Rights: Legal Deposit; Only available on premises controlled by the deposit library and to one user at any one time; The Legal Deposit Libraries (Non-Print Works) Regulations (UK).
• Access Usage: Restricted: Printing from this resource is governed by The Legal Deposit Libraries (Non-Print Works) Regulations (UK) and UK copyright law currently in force.